FastQCFastQC Report
Mon 25 Nov 2019
000000000-CF4LF_l01_n01_EMP_11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-CF4LF_l01_n01_EMP_11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64529
Sequences flagged as poor quality0
Sequence length251
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA1065616.51350555564165No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA774311.999256148398395No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA41606.446713880580825No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA38886.025197973004386No Hit
GACGAACCAAGCGAACGTTATTCGGAATTACTGGGCTTAAAGCGCGTGTA33075.12482759689442No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA12721.9712067442545211No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA11451.774396007996405No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGAGCGTA11371.76199848130298No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTCACTGGGTTTAAAGGGTGCGTA8851.3714763904601033No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATCACTGGGCGTAAAGCGCACGTA8751.3559794820933224No Hit
TACGAAAGGGGCTTGTGTTATTCGGTTAAATTGGGCGTAAAGGATTCTAA8321.2893427761161649No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA8071.2506005051992128No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGTTCGTA7331.1359233832850346No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA6280.9732058454338358No Hit
TACGTAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA6150.9530598645570207No Hit
TACGAAGGGTGCTAGCGTTGTTCGGAATGACTGGGCGTAAAGGGTGCGTA5370.8321839792961303No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGTTGCAGTTAAAAAGCTCGTA5170.8011901625625688No Hit
TACGAAGGGGGCTAGCGTTGTTCGGATTTACTGGGCGTAAAGCGCACGTA3810.5904322087743494No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGGGTGCGTA3440.5330936478172604No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGCGCGTA3410.5284445753072262No Hit
TACGGAGGGAGCTAGCGNTGTTCGGAATTACTGGGCGTAAAGCGCACGTA3360.5206961211238358No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCACGTA3300.5113979761037674No Hit
TACGGAGGGGGCTAGCGTTATTCGGAATTACTGGGCGTAAAGCGCACGTA3230.5005501402470207No Hit
TACATAGGGTGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGAGCTCGTA3220.49900044941034266No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATGACTGGGCGTAAAGGGCGCGTA3160.4897023043902741No Hit
TACGAAGGGGGCTAGCGTTGCTCGGAATTACTGGGCGTAAAGGGAGCGTA3100.48040415937020564No Hit
TACGAAGGGTGCAAGCGTTACTCGGAATTACTGGGCGTAAAGCGTGCGTA2940.45560910598335636No Hit
TACGTAGGGTGCAAGCGTTGTCCGGATTTATTGGGCGTAAAGAGCTCGTA2680.4153171442297262No Hit
TACGTAGGGTCCGAGCGTTGTCCGGAATTACTGGGCGTAAAGAGCTCGTA2270.3517798199259248No Hit
GACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGAGTGTA2240.3471307474158905No Hit
GACGAACCGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA2190.3393822932325001No Hit
TACAGAGGGTGCGAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA2160.33473322072246586No Hit
GACGGAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGAGCGCGTA2080.3223356940290412No Hit
TACGTAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA2070.3207860031923631No Hit
GACGAAGGATCCAAGCGTTGTCCGGATTTACTGGGTTTAAAGGGTGCGTA2060.319236312355685No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA2030.3145872398456508No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGTACGTA1930.29909033147886993No Hit
TACAGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGTA1840.2851431139487672No Hit
TACGAAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA1700.26344744223527405No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGCA1670.2587983697252398No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1630.25259960637852746No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGCA1510.2340033163383905No Hit
TACAGAGGGTGCAAGCGTTAATCGGATTTACTGGGCGTAAAGCGTGCGTA1470.22780455299167818No Hit
GACGAGGGATCCTAGCGTTGTTCGGAATCATTGGGCGTAAAGCGGGTGTA1460.22625486215500007No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGTA1410.21850640797160967No Hit
TACGAGGGGAGCAAGCGTTATTCAAAAAAACTGGGCGTAAAGAGTTTCTA1340.2076585721148631No Hit
TACGTAGGGTCCGAGCGTTGTCCGGAATTATTGGGCGTAAAGGGCTCGTA1330.206108881278185No Hit
GACGAACCGTGCGAACGTTGTTCGGAATCATTGGGCTTAAAGGGCGCGTA1290.19991011793147268No Hit
CGAAGCTAACGCGATAAGCACCCCGCCTGGGGAGTACGGCCGCAAGGTTA1270.1968107362581165No Hit
TACGGAGGGTGCAAGCGNTAATCGGAATTACTGGGCGTAAAGCGCACGTA1270.1968107362581165No Hit
TACAGAGACTGCAAGCGTTATTCGGATTCACTGGGCGTAAAGGGTGCGCA1250.19371135458476033No Hit
TACGGAGGATCCAAGCGTTATCCGGAATCATTGGGTTTAAAGGGTCCGTA1230.19061197291140416No Hit
TACGGAGGGTGCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTCCGTA1170.18131382789133568No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGCTCGTA1170.18131382789133568No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCACGTA1170.18131382789133568No Hit
TACGGAGGGTGCAAGCGTTATCCGGAATCACTGGGTTTAAAGGGTGCGTA1160.1797641370546576No Hit
TACGAAGGGGGCTAGCGTTACTCGGAATTACTGGGCGTAAAGGGCGCGTA1130.17511506454462336No Hit
GACGAGCTCTCCAAACGTTATTCGGTATCACTGGGCTTAAAGAGTTCGTA1120.17356537370794525No Hit
TACGTAGGGTGCGAGCGNTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1110.17201568287126717No Hit
TACGAAGGGTGCTAGCGTTGTTCGGAATGACTGGGCGTAAAGGGCGCGTA1080.16736661036123293No Hit
TACGAACTGTGCGAACGTTATTCGGAATCACTGGGCTTAAAGGGTGCGTA1080.16736661036123293No Hit
TACGGAGGGGGTTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGTA1070.16581691952455485No Hit
GACGAACCAAGCGAACGNTATTCGGAATTACTGGGCTTAAAGCGCGTGTA1000.1549690836678083No Hit
TACGAGAGGAGCGAACGTTGTTCAAAATAACTGGGCGTAAAGGGTGAGTC930.1441212478110617No Hit
TACGGAGGGGGCTAGCGTTGTTCGGAAATACTGGGCGTAAAGCGCACGTA900.13947217530102743No Hit
TACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGTA890.13792248446434938No Hit
TACGGAGGGAGCTAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA880.13637279362767127No Hit
TTCCAGCTCCAATAGCGTATATTAAAGTTGCTGCAGTTAAAAAGCTCGTA830.12862433944428087No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGCGCGCA790.12242557609756853No Hit
TACGGAGGGTGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA750.1162268127508562No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGAGCGCA690.10692866773078771No Hit
TACGAAGGGGGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCGCGCA670.10382928605743155No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGGGTGCGCA660.10227959522075346No Hit
TACGAGAGGTGCAAGCGTTATTCGGAATAATTGGGCGTAAAGGGTGCGTA660.10227959522075346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTGCA20450.0244.815527
CGAACCG400.0244.81553
GGATGCA201.5562728E-7244.81557
GATCCTA301.6370905E-11244.81558
GATCCAA800.0244.81558
GGATCCA800.0244.81557
GTGGCAA151.5173044E-5244.81558
GAGGTCT251.6043487E-9244.81555
CGTAGGA251.6043487E-9244.81553
TACGAGG301.6370905E-11244.81551
GAGCGAG151.5173044E-5244.81559
AGACCTC151.5173044E-5244.81556
CAAATAG151.5173044E-5244.8155245
GTCCGAG400.0244.81559
GGTGCAG100.0014763083244.81558
AAGGGGC800.0244.81556
ACCGTGC400.0244.81556
ACGTACT201.5562728E-7244.81552
GAACCGT400.0244.81554
AGCTCCA1900.0244.81555
Produced by FastQC (version 0.11.5)