FastQCFastQC Report
Mon 23 Sep 2019
000000000-C9WWL_l01.4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C9WWL_l01.4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7728509
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGAGGCGGAGACGGTGAGGCTATACGCACCTT164910.21337880307831691No Hit
CTGTCTCTTATACACATCTGAGGCGGAGACGGTGCGTTGGTTGCGTGGAG102210.13225060616478548No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA88340.11430406563542851No Hit
ACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAG79730.10316349505447946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGCAC20650.038.67312641
TATACGC20850.038.30216239
CTATACG21050.037.9382438
ATACGCA22300.036.0089740
TCGCATA7800.034.41025540
ACGCACC23900.033.59832442
ACGGTGA25150.032.2783329
ATCGCAT8350.032.1437139
GAATCGC14150.031.87279325
CGGTAAT8550.031.39181334
TAGACCG6450.031.038764
GACGGTG42600.030.93427328
AGACGGT42900.030.66666627
CGTTATG9200.030.60869612
TAGTCGA6700.030.53731333
CGCTTGA17550.029.83475729
ATCGCTT17800.029.78651827
CCGGTAA9100.029.49450733
GTTGCGT17450.029.24928540
GTATACG7600.029.23684144