FastQCFastQC Report
Mon 23 Sep 2019
000000000-C9WWL_l01.1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C9WWL_l01.1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7728509
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGCCGTCCTCGATCGCATTACTCGCCTGTCCC160340.20746563146914884No Hit
ACCAACAGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAG77630.10044628271766262No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATT21050.039.08788731
ACGCAAT2850.034.73684324
CGCATTA23750.034.5515832
CGGTAAT9350.033.6470634
TACTCGC24750.033.24444637
AGCGTTA9450.032.82539710
ACGCTAC10400.031.73076833
ACTCGCC26000.031.73076838
CGATAGT4250.031.5764740
TTACTCG26100.031.52490436
GATCGCA31300.031.4185329
TCGCATA10000.031.2440
CACGCTA8750.031.17714332
GAATCGC10250.031.1219525
TAGACCG8250.030.9333324
CCGGTAA10200.030.6274533
CGTTATG10050.029.9900512
TAGTCGA7600.029.52631633
GTATACG8750.029.41714344
ATCGCAT28800.029.33333430
Produced by FastQC (version 0.11.5)