FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650879
Sequences flagged as poor quality0
Sequence length251
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG11300.057.45575245
GATCGGG7700.055.6818161
TCGGGAG7250.054.0689663
GCGTCGG7600.051.578945245
GAGGGGC6550.044.8854949
ATCGGGA10000.041.6499982
CGGGAGA10450.041.0287064
CGGTGGG13200.037.121212245
GGGAGAG16550.029.607255
GAGAGGG16800.024.7916667
AGAGGGG16750.024.1343278
GGAGAGG18150.021.5977956
TCGCCGG10250.020.317072245
GAGCGGC7909.7528755E-817.0569619
GAACGGG8202.6864818E-614.9390241
GGAGAGC12554.3837645E-1014.6414336
AACGGGA12651.1238444E-712.5889322
GAGAGCG14103.5106495E-812.163127
AAAGAGG51050.011.998041245
GGGGGGC12450.011.21686740-44
Produced by FastQC (version 0.11.5)