FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800647
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTTGCTGAAGCTGGAGTATCTCCCTTTTGTTTTTGGAGTTGTGAGGCA38330.4787378207874382No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGTTTTAGGTG30530.3813166101915076No Hit
ACAGGCTTGCATATCAGAGCTAGAAGAGTCAGTCTGCCTTTTACATAACT23850.2978840862452492No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGACGATT22540.28152231882465056No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGT20700.25854090504304644No Hit
TCTCTTCTCCCCCAAGCCTGTTTTCCAGCTGATCCACCAGAGGCATCATC18730.23393580441817682No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCACCC18460.23056353174370228No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTTGGTTA18240.22781575400894527No Hit
GGACCTTCCCCAGCTTCATTACAAGCTTGAATACAGAATTTATAGGATGC17840.22281979449120526No Hit
GTCTGCAAGCCGAATTTGGATGTTTGTGGTAGGCTCTGATTCGTTGATTA17450.2179487339614087No Hit
AGAGACTTCCTTGATTCGACCATCTTGTCTAAAGGAGTATGCTTTAGGTG15100.18859747179468606No Hit
GCCATCTCCTTAGGTCCTCCTTTCTTGCCACTCTCTTGTCCATTTCCTTT14370.1794798456748105No Hit
CTGAGGTGAAGTGACTGCCTGGTGGAGTTGCTGCAGCACGCTCCGACATG13200.16486666408542092No Hit
CAATTGCCCACACCAACTCCTCCAGCTTGGCTTGTCTGCTCTTCTCATCC12790.1597458055797374No Hit
ACTCCTTCACTTTCCAGAAATCTTCCTGGTGGTCGTTCTTAAGGATGATT9560.11940343247398666No Hit
TCTTAAGATCAAGGGTAGCTGCATTATTGACTTGGCCAATTTGTCGGTTA9540.11915363449809967No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTAGG4350.0188.678168
AGACTTC7500.0184.566683
GACTTCC7500.0184.566684
GAGACTT7600.0183.752
AGCCGAA4900.0175.08
TCCCCAG6300.0169.166667
CAAGCCG5100.0168.137256
CTTAGGT4950.0163.333339
TGCCCAC5500.0160.363635
CCCCAGC6600.0159.621228
AGAGACT8750.0159.599991
GACCTTC6850.0157.372252
AAGCCGA5550.0156.711727
CCTTGAT8850.0156.412439
GCCCACA5650.0156.106196
TCAAGGG7600.0148.289479
GATCAAG7800.0146.057687
TCCTTAG5700.0143.991237
TTGCCCA6500.0139.461554
TTCCCCA8000.0133.218756
Produced by FastQC (version 0.11.5)