FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252772
Sequences flagged as poor quality0
Sequence length251
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG15450.076.1165245
GCGTCGG14000.057.749996245
GATCGGG9200.047.934781
TCGGGAG10700.041.214953
GAGGGGC8950.041.061459
ATCGGGA13300.035.9210552
CGGGAGA14200.029.3309884
GGGAGAG22300.028.5650235
CGGTGGG17450.027.378222245
AGAGGGG21750.025.3448288
TCGCCGG14650.022.576792245
GGAGAGG25800.021.8410856
GAGAGGG24700.021.8218617
AAAGAGG78250.015.811501245
GGGGGGC15800.015.5063340-44
CGGGAGG7653.721399E-412.8104584
GGGGGCC8550.012.32163740-44
GATAGGG15951.196895E-912.2884021
GGGGGCG15400.011.29545540-44
TTGGGAG24950.011.2925843
Produced by FastQC (version 0.11.5)