FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_S4-joe.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_S4-joe.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2127643
Sequences flagged as poor quality0
Sequence length251
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG13000.064.07693245
GCGTCGG11750.041.702126245
CGGTGGG16650.035.31531245
TCGCCGG13000.022.615385245
GAGGGGC7401.6875963E-514.8986489
GTCGTGG11255.278449E-611.977778245
AAAGAGG70350.011.666667245
ACGAGCG9750.002832950310.051282245
AGAGTGG19452.871584E-79.447301245
AGAGGGG17105.182466E-47.8801178
CTAAGAC14150.00670731357.79151961
AGAGCGG14600.0088746587.55137245
CCTCACC19200.00177869247.018229245
CACACAA54052.004872E-75.4394083245
TAAGGAT34157.6424005E-45.3806742
AGACACA39701.8692994E-45.245592245
GTTTAAA50008.162742E-65.1451
GGAAAGG31100.0082226745.120579245
Produced by FastQC (version 0.11.5)