FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1809873
Sequences flagged as poor quality0
Sequence length251
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG13150.068.93536245
GCGTCGG10100.048.51485245
TCGCCGG12200.033.135246245
CGGTGGG14750.020.76271245
AAAGAGG59050.013.899238245
GTCGTGG9259.351814E-613.243243245
GAGCGTG10100.00379278289.70297245
AGAGTGG14901.1698867E-49.043624245
GGCTCCC12750.0026225938.6470599
GGAAAGG30151.411069E-46.0945272245
CTTTACC33155.12367E-45.54298641
Produced by FastQC (version 0.11.5)