FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930999
Sequences flagged as poor quality0
Sequence length251
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG8400.055.4165731
GAGGGGC8650.052.3987589
TCTCGGG15700.050.717785245
GCGTCGG9600.040.83432245
TCGGGAG10250.040.634083
ATCGGGA12300.037.8454672
TCGCCGG12700.032.796066245
GGGAGAG19750.031.6328585
GAGAGGG18650.030.2144247
AGAGGGG22300.028.5649748
CGGGAGA13700.027.7189314
GGAGAGG21850.025.228796
AAGGGGC10050.020.7213579
GGTCGGG5357.660201E-720.6074431
GGGGGGC13750.019.42178540-44
GAGCGGC9751.9281288E-1017.5897149
AGAGCGG14450.016.9549888
GTCGGGA6807.5727075E-616.213212
TCCGGAG7751.4935067E-615.8064253
CGGTGGG16300.015.78259245
Produced by FastQC (version 0.11.5)