FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n02_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n02_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1588937
Sequences flagged as poor quality0
Sequence length251
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGGG35650.020.96073245
CGGTGGG35900.019.449862245
AGAGTGG21100.018.5782245
AAAGAGG46950.015.915868245
GGAAAGG18851.1277734E-1011.697613245
CGGAAGG13753.322184E-711.581819245
GCGTCGG58400.011.536814245
GATTACC7950.007191596610.7861634
AGAGCGG36500.09.397261245
GGAATAA12200.00175688229.0368854
GTCGTGG35550.08.959212245
TCGCCGG59350.08.668914245
ATACGTG13550.0045437048.1365313
GAAGAGG35851.2244709E-76.8340306245
ATTGTGG19900.00259340626.7713571
AGAGTGA18550.0098914936.6037736245
GAGAGAA20450.00345254626.589242245
GGGCAGG25300.0048497665.81027652
GCTGTTT25350.0049572445.7988164
CGGGGGG28600.00306914425.5681815245
Produced by FastQC (version 0.11.5)