FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n01_wt.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n01_wt.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650879
Sequences flagged as poor quality0
Sequence length251
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC47770.2893610010182454TruSeq Adapter, Index 7 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGAC3300.00690461618.5606067
GAATCGG10005.113179E-915.9249991
AATCGGA14456.3145126E-711.0207612
AGTCCGG12350.00196353468.9271251
GGGCGCC3200.00235124867.656249520-24
ATATTCG22150.00109053616.63656855
CCGCCCC22500.0093257625.98888879
GCCCACG5350.008990515.4953265225-229
CAGCGGG6300.0095522215.05555665-69
ATGATTT65858.5612555E-85.0227793
Produced by FastQC (version 0.11.5)