FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences800647
Sequences flagged as poor quality0
Sequence length251
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTCGGGTCCACTAAAAATCTCTAGTGTATCAGAAGTAATGAAAGAATC38620.4823598914377997No Hit
GAATGGACGGTTTGTATCCCTATACAGAGGACCATGTCATACATACAAAG38460.4803615076307037No Hit
CCAAGTATCTCAAATGCCCGGAATGCAGAGCTCCGGCTGCGATGGGGCCA32610.4072955996837557No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAACATACT32200.4021747411780722No Hit
TGGATGGAAACTTAAGCGTTGTTGATGATATTCCAGAATTCAAAGACTTG30500.3809419132276771No Hit
GAGTGACAGCGAAGAAGAATTTTTTGAATGCCTAAGTGATACTGAAGAAC21480.2682830261026395No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCAACAAATTGGCCAAG20350.25416944046502393No Hit
ATCTGCCTCATCACACCCATGTCTTTCATGAAGGCATGTCGGAGCGTGCT19950.2491734809472839No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCAACG18700.2335611074543463No Hit
GAGATCCCCTGGTCCCAAACCAGAAGGGCTACTGGCACAGTTATGTAAAA16250.20296085540818862No Hit
TCTTCCTTCTAATGATGAGCCCATCCTTTCATTTTCACCTAAAGCATACT15660.1955918151195221No Hit
ATCTTCCTTTTTGATACCCAGCATTTTATAAGTAAAGAGAGAGCAGGAGC12060.15062817945986184No Hit
AGAAAGTGTTGTTGGTTCAGTGACACAACTTAACCGACAAATTGGCCAAG10820.13514070495486774No Hit
CCAACAGGCAGAAAATGAAGCCAAAGCCAGCTCTTCCATCTTAATCGACG8170.10204247314984008No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTCC4250.0193.117655
TGGACGG5550.0192.027024
CCCCTGG3450.0184.637686
GGTCCAC4450.0184.43827
GAGTGAC4500.0182.388891
TCGGGTC4500.0182.388894
GGGTCCA4550.0180.384616
CGGTTTG6050.0176.157038
GGATGGA5550.0169.954962
GACAGCG5100.0160.931385
GATCCCC3900.0160.19233
TCCCCTG4000.0159.255
ACGGTTT6700.0159.067177
GTGTTGT7500.0156.800026
CTGGTCC4000.0156.18759
AGTGTTG7550.0155.761585
TGACAGC5350.0153.411214
GTGACAG5400.0151.990743
ACAGCGA5450.0150.596336
GCGAAGA5550.0147.882899
Produced by FastQC (version 0.11.5)