FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n01_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n01_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2252772
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC75130.33350023881688867TruSeq Adapter, Index 6 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG13800.020.4166661
AATCGGA21750.012.3908042
CCAGTCC16654.035472E-58.828829245
GGCTCCC13350.00397520938.2584279
CACATCC23402.73832E-46.805556245
CCACTCC22900.00159001446.419214245
TCCCGGC4500.0093371665.98888995-99
AGAAGCT44203.0678835E-55.2658371
Produced by FastQC (version 0.11.5)