FastQCFastQC Report
Wed 13 Nov 2019
000000000-C7PW4_l01_n01_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C7PW4_l01_n01_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930999
Sequences flagged as poor quality0
Sequence length251
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC90310.4676853794331328TruSeq Adapter, Index 4 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGG11106.91216E-1116.5540141
AATCGGA16401.8535502E-911.9511912
ATTTAGC26350.00115512996.04362871
ATATGCT34551.4654506E-45.67292451
TAGGTAT26650.0086186135.5159353
CACTTCC29350.00416779845.4260225245
CTGTTAT29950.00529007155.3171831
CCGTGGC9856.2213494E-65.223338620-24
CACACTC30900.00762323065.1538434245
ATAAACG31500.0095409955.05554341
TACACAA44051.6282665E-45.0057936245
Produced by FastQC (version 0.11.5)