FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516518
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC14830.28711487305379485No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6980.13513565838944624No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGCG301.2999335E-463.33941
TTAGCCG301.3005565E-463.3332673
TAGCCGG301.3005565E-463.3332674
AATTGCC656.693881E-1058.4671361
AATTCGC405.382865E-447.504551
AATTCAC1100.047.504551
AATTCCG652.5848894E-643.8503531
AATTGGT1051.024091E-940.7181851
AATTAAT1208.731149E-1139.5871281
AGGTTCG308.290219E-539.5832956-57
AATTGTC905.685597E-736.9479871
AATTTGG801.0757536E-535.6284141
AATTGGA702.050618E-433.931821
AATTCGG851.6294012E-533.5326231
AATTCTA753.0743913E-431.6696991
CACGACT300.003950787731.66663470-71
ACTCGAT300.003950787731.66663482-83
AATTCTT2300.030.9812281
CCGGGCG856.402404E-427.9411477
AGCCGGG700.00838740927.142835
Produced by FastQC (version 0.11.5)