FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm228RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm228RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730470
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG2350.052.5531921
ATCGGGA3350.034.029852
CGCCAAT856.4049265E-427.9411751
CGGGAGA5550.025.6756764
GTTCGGG1703.1463805E-622.352941
GAGAGGG8650.021.9653177
GAGAGCG3750.021.5333337
TTCGGAG1353.7941188E-421.111113
TCGGGAG6000.020.5833323
AACGGGA2108.598872E-720.3571432
AGAGGGG9650.019.6891218
GAGGGGC9050.019.419899
ATTCGGA1801.23254E-418.4722212
AGGGGCG5150.018.44660210-11
GGGAGAG11400.017.5000025
CATGCGT850.001787876616.76470648-49
GAACGGG2555.5006512E-616.7647061
GGAGAGG12000.016.2291666
GGGGTAT1850.00314185315.4054061
AGAGCGG2801.3363442E-515.2678568
Produced by FastQC (version 0.11.5)