FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm227RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm227RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG3200.043.0468751
ATCGGGA3300.041.7424242
CGGGAGA6050.019.6280984
TCGGGAG5050.018.811883
GAAAGCG3208.260031E-917.81257
TCGGGAA3151.338376E-716.5873013
AGGGGGG7750.016.24193438-39
CGGGTTA1105.7026657E-415.11363660-61
AAAGCGG2851.5804695E-515.0000018
CGAAATC2000.00527631514.252
GGGGAAT4704.2109605E-914.1489361
TAGGGGG4200.014.13690536-37
ATCGAGA2802.2815922E-413.5714296
ATGTCGT1050.007293747313.57142856-57
AGAGCGG3901.5019032E-613.3974358
GAGAGGG10900.013.0733957
CGGGTGT2200.0099125912.9545461
AGGGGCG5150.012.912621510-11
GAGATGT5806.037226E-912.2844833
CGGGAAA3952.5166473E-512.0253164
Produced by FastQC (version 0.11.5)