FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm226RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm226RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG3250.042.3846171
ATCGGGA3450.039.9275362
TCGGGAG6100.022.5819683
GAGAGCG4100.020.8536597
AGAGCGG3451.8189894E-1220.6521748
CGGGAGA6250.019.764
CTCGCAT1759.932415E-519.01
TAGTCGC650.007564481318.26923272-73
CGCGGTT1350.00939820517.5925921
GCACGTT2355.1315365E-516.1702141
GAAAGCG2406.1439125E-515.8333347
GATTGGG3755.8338628E-815.2000011
AGGGGCG5000.015.210-11
ATCGGGG3500.014.92857248-49
GACCTAC2100.007289679313.5714285
GAGAGGG10300.013.3737877
AGAGGGG10300.013.3737878
AGGGGAA11100.013.26576620-21
TCGGGGG5800.013.10344850-51
GGGAGAG13650.012.8754575
Produced by FastQC (version 0.11.5)