FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm225RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm225RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822460
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA2750.029.3636362
GATCGGG2650.028.6792431
GTTCGGG1201.7102403E-423.751
AAAGCGG2108.6015825E-720.3571438
GATTGGG3603.6379788E-1219.7916661
GGGTATA3600.018.47222134-35
CGGGAGA5200.018.2692324
ACGGACC1350.00939819317.5925925
ACGGGAG1952.274323E-417.0512833
TCGGGAG4751.8189894E-1217.03
ATTGGGA4551.5825208E-1015.6593412
TCGGGAA3051.7400489E-615.57377053
GGTATAA2900.015.56034534-35
AACGGGA2154.78538E-415.46511652
TACTCTA1850.003142302215.4054069
CGGGGAA2508.7059976E-515.21
ATTGCGG1105.70266E-415.11363676-77
GAGAGGG8850.015.0282497
CGAAGTG1950.004460022314.6153853
GAGAGCG4552.6666385E-914.6153847
Produced by FastQC (version 0.11.5)