FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm218RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm218RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718594
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGA1701.12160706E-725.1470582
GATCGGG2002.0512744E-823.751
CTCGCAC1750.002168672916.2857151
CGCCAAC1800.0026173315.8333331
CCGGGAT1850.00314178715.4054061
CGAGATT1950.00445929414.6153852
AGGGGCG3450.014.45652210-11
GCGCGAC1000.005278846214.2544-45
AATCGGG1000.005278846214.2548-49
CGGGGAT2500.001498179613.2999991
TTCGGCG1300.002014301512.78846246-47
AGAGGGG7450.012.75167758
GAGGGGC7601.8189894E-1212.59
AGAGCGG2700.002671841312.31481558
ACAATCC2750.003065686212.0909093
TACAAAC3551.2434626E-412.0422539
CGGGAGA4002.8613378E-511.8754
CGGGCTT3258.0142135E-411.6923071
ATTCGAG1851.8304588E-411.55405490-91
CGGGTAG1659.118299E-411.51515214-15