FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n02_ALm217RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n02_ALm217RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664566
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG3550.056.1971861
ATCGGGA3750.051.9333342
CGGGAGA5450.036.6055034
TCGGGAG5250.035.2857133
GAGAGCG3900.028.012827
GAGAGGG8050.025.9627327
CGTTAAG950.0012260125.0000026
AGGGGCG4000.023.7510-11
AGAGCGG3200.023.7499988
GAGGGGC7950.023.3018869
GGGAGAG12600.022.6190455
GGGTATA2650.021.50943434-35
AGAGGGG10300.020.2912628
GATTGGG2902.4338078E-919.6551721
GATAGGG2001.283402E-519.01
ATTGGGA3105.5679266E-918.3870962
GGAGAGG11200.018.2366096
ATAGGGA2403.0861265E-617.81252
AGGGTAT3650.017.56849332-33
AGGGGAA9200.017.55434820-21
Produced by FastQC (version 0.11.5)