FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences516518
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20860.40385814240742823No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8560.1657251054174298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTAT156.6158467E-495.08
CGATCAT2900.090.0862052
TGCATGC9000.088.138894
TCGATCA3000.087.0833361
CAACCCA4600.083.6413041
ATCATGC5800.082.7155154
ATGCATG7750.082.1290363
AACCCAT4750.081.02
CATGCAC3050.079.426226
GCATGCC3700.078.3108145
CATGCGT750.076.06
CATGCAT8500.074.882352
CGATCCA1800.073.8888851
ATGCACG409.051291E-871.257
TTGCATG2200.069.090913
GATCCAT2050.067.195122
GCATGCG1150.066.086965
ATCCATG1950.065.769233
TGCATCA3400.065.6617661
GCATGCA9950.063.969851
Produced by FastQC (version 0.11.5)