FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm228RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm228RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences730470
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT43040.5892096869139047TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC6350.041.88976746-47
CTCGTAT6300.040.71428744-45
TATGCCG6550.039.88549448-49
CGCGAAA6550.039.16030536-37
TCGTATG6650.038.21428344-45
CGAAATC6850.036.75182338-39
GCGAAAT6850.036.0583936-37
TCTCGTA6850.036.0583942-43
AATCTCG6850.036.0583940-41
ACTCCGC7400.035.30405432-33
CCGCGAA7000.035.28571334-35
ATCTCGT7400.034.66216342-43
TCCGCGA7450.034.42952734-35
ATGCCGT7600.034.062548-49
CACTCCG7450.033.79194630-31
CTCCGCG7700.032.38636432-33
CCGTCTT8650.030.75144452-53
TGCCGTC8650.030.47687750-51
GTATGCC8350.030.149746-47
GCCGTCT8650.029.37861350-51
Produced by FastQC (version 0.11.5)