FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm227RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm227RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT38550.4681005560136046TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4750.043.044-45
CGCGAAA4750.041.536-37
TATGCCG4950.040.30303248-49
CTCGTAT5000.039.42500344-45
CGTATGC5150.038.73786546-47
AATCTCG5500.035.84090840-41
CGAAATC5650.034.8893838-39
TCTCGTA5750.034.69565242-43
GCGAAAT5800.033.9870736-37
CCGCGAA6000.033.64583634-35
ATCTCGT6050.032.97520442-43
TCCGCGA6350.032.16535634-35
ACTCCGC6300.031.66666832-33
ATGCCGT6700.030.83955448-49
GTATGCC6650.030.35714346-47
CCGTCTT7100.028.43309852-53
CACTCCG7100.028.43309830-31
CTCCGCG7300.027.6541132-33
GCCGTCT7700.026.21753350-51
TGCCGTC7600.025.93750250-51
Produced by FastQC (version 0.11.5)