FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm226RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm226RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences823462
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT56350.6843060153352553TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA6450.043.08139436-37
TCGTATG6400.042.6757844-45
CGTATGC6800.041.562546-47
TATGCCG6750.041.16666848-49
CTCGTAT6650.040.71428344-45
CCGCGAA7000.039.35714334-35
TCCGCGA7150.039.19580534-35
GCGAAAT6950.038.95683336-37
AATCTCG6900.038.89492840-41
TCTCGTA7000.038.33928742-43
ATCTCGT7300.037.08904342-43
CGAAATC7500.036.41666838-39
CACTCCG7950.035.2515730-31
ATGCCGT8200.033.30792648-49
ACTCCGC8650.032.6734132-33
GTATGCC8550.032.546-47
GCCGTCT8550.031.66666650-51
CTCCGCG8950.031.5782132-33
CCGTCTT9000.030.08333452-53
TGCCGTC9550.028.8481750-51
Produced by FastQC (version 0.11.5)