FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm225RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm225RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences822460
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTAT52010.6323711791454903TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6000.040.77083248-49
TCGTATG6000.040.77083244-45
CGTATGC6300.038.82936546-47
CGCGAAA6500.038.036-37
CTCGTAT6400.037.85156244-45
GCGAAAT6700.036.8656736-37
AATCTCG6800.035.97426240-41
TCTCGTA6900.035.797142-43
CCGCGAA7150.035.20979334-35
TCCGCGA7150.034.54545634-35
CGAAATC7150.034.21328738-39
ACTCCGC7500.033.2532-33
ATCTCGT7400.032.7364942-43
ATGCCGT7700.032.69480548-49
GTATGCC7550.032.4006646-47
CACTCCG8100.030.78703730-31
CCGTCTT8200.030.70121852-53
CTCCGCG8500.029.89705832-33
TGCCGTC8450.029.51183550-51
GCCGTCT8350.029.29640850-51
Produced by FastQC (version 0.11.5)