FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm220RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm220RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences648556
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT23970.36959028981306163TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC3450.041.30434846-47
TATGCCG3650.039.04109648-49
CTCGTAT3700.037.87162444-45
TCGTATG3600.035.62500444-45
TCTCGTA4250.030.17647242-43
TATGATC5200.028.77403838-39
GTATGCC4700.028.29787346-47
CGGCTAT5050.028.21782134-35
ACCGGCT5150.028.1310732-33
ATCTCGT5050.027.74752442-43
AATATCG350.008391960527.14285910-11
TCACCGG5350.027.0794430-31
CCGGCTA4850.026.93299132-33
GCCGTCT5200.025.12019350-51
CCGTCTT5700.024.99999852-53
GTCACCG5400.024.62963128-29
TGCCGTC6150.024.32926850-51
ATGCCGT5400.024.18981748-49
GCTATGA6000.023.7536-37
GATCTCG5700.022.540-41
Produced by FastQC (version 0.11.5)