FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm219RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm219RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720564
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT28730.3987154506747493TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG2350.037.39361644-45
TATGCCG2800.032.23214348-49
CGTATGC2750.031.95454646-47
CTCGTAT2700.031.6666744-45
TCTCGTA3250.027.03846242-43
GTATGCC3600.026.38889146-47
ATGCCGT3800.023.12500248-49
CCGGCTA4100.022.01219632-33
GCCGTCT4100.021.43292850-51
CGGCTAT4300.020.98837334-35
TATGATC4300.020.43604738-39
ATCTCGT4200.020.35714342-43
GTCACCG4400.019.97159228-29
GCACGAT1707.9485995E-519.5588251
CCGTCTT4500.019.52777752-53
ACCGGCT4800.019.29687532-33
TCACCGG4700.019.20212730-31
TGCCGTC5000.018.05000150-51
GCTATGA5200.017.812536-37
CGTCTTC5500.015.97727352-53
Produced by FastQC (version 0.11.5)