FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm218RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm218RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences718594
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT34910.4858097896726107TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3800.041.87544-45
CTCGTAT3750.040.53333744-45
CGTATGC4200.037.88690646-47
TATGCCG4250.037.44117448-49
TCTCGTA4400.035.08522842-43
ATGCCGT5350.031.07476448-49
CGGCTAT5400.030.78703934-35
ATCTCGT5000.030.442-43
CCGGCTA5350.030.18691432-33
ATCGGAA11000.028.9318182
CCGTCTT5850.028.82478752-53
GCCGTCT5800.028.66379450-51
ACCGGCT5650.028.58407232-33
GATCGGA11300.028.1637171
GTATGCC5700.027.91666846-47
TCGGAAG12050.026.4107883
CGGAAGA12250.026.3673464
TCACCGG6150.026.26016230-31
TGCCGTC6400.025.9765650-51
GAGCACA12850.025.8754869
Produced by FastQC (version 0.11.5)