FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm217RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm217RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences664566
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCTATGATCTCGTAT51140.7695247725583313TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC7050.042.44680846-47
TCGTATG6950.042.03237544-45
CTCGTAT7200.040.57291444-45
TATGCCG7350.040.39115548-49
CGCGGAT756.9178786E-638.01
TCTCGTA7550.037.43377742-43
ATCTCGT7850.036.91082842-43
GTATGCC7900.036.67721646-47
CGGCTAT8200.036.493934-35
CCGGCTA8000.036.2187532-33
CCGTCTT8200.035.9146352-53
TATGATC8450.034.57100738-39
GCCGTCT8550.033.8888950-51
ATGCCGT8950.032.9050348-49
ACCGGCT9250.032.6081132-33
GTCACCG9000.032.45833628-29
TCACCGG9300.032.1774230-31
AGAGCAC19000.032.0000048
GAGCACA19000.031.7500029
CGTCTTC9450.030.66137752-53
Produced by FastQC (version 0.11.5)