FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm212RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm212RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences736649
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT30740.4172950754022608TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4000.040.37546-47
TCGTATG3950.039.68354444-45
TAATGCG4350.037.1264434-35
TATGCCG4550.036.01648748-49
CTCGTAT4550.034.9725344-45
CTACCGT851.6314105E-533.529418
TACCGTA902.4117948E-531.6666689
AATGCGC5000.031.34999834-35
GTATGCC5450.030.06880846-47
ATCTCGT5300.030.02358642-43
TCTCGTA5300.030.02358642-43
ATGCCGT5600.028.4151848-49
CTAATGC5750.027.26086832-33
ATGCGCA5950.027.14285936-37
TGCGCAT6000.026.12499836-37
CCGTCTT6500.025.57692352-53
GCGCATC6250.025.46000138-39
CATCTCG6300.024.88095340-41
GCCGTCT6400.024.86328350-51
CGCATCT6500.024.11538538-39
Produced by FastQC (version 0.11.5)