FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm211RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm211RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences779610
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT31520.40430471646079447TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC4000.037.4062546-47
TCGTATG4000.036.812544-45
TATGCCG4200.035.62548-49
TAATGCG4250.035.20588334-35
CTCGTAT4250.034.6470644-45
TCTCGTA4600.032.0108742-43
AATGCGC5000.030.434-35
CCGTCTT5050.028.68811852-53
TGCGCAT5250.028.0476236-37
GCGCATC5500.027.63636438-39
ATCTCGT5550.026.53153242-43
ATGCGCA5650.026.48230236-37
GCCGTCT5600.025.87053550-51
CTAATGC6150.025.10162732-33
CGCATCT6000.024.93750238-39
ATGCCGT5850.024.76495748-49
GTATGCC6050.024.73140346-47
CGTCTTC6150.023.9430952-53
CATCTCG6150.023.9430940-41
ACTAATG6850.021.84306532-33
Produced by FastQC (version 0.11.5)