FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm210RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm210RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences788379
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT40140.5091459818183893TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG4800.041.0677144-45
CTCGTAT4850.040.1546444-45
CGTATGC5000.039.42546-47
TATGCCG5300.037.19339848-49
TCTCGTA5250.036.19047542-43
TAATGCG5550.035.94594634-35
AATGCGC5800.033.9870734-35
ATCTCGT5750.033.45652442-43
CGCATCT6200.031.41129138-39
TGCGCAT6600.030.58712236-37
ATGCGCA6600.030.58712236-37
GCGCATC6500.030.32692338-39
CCGTCTT6650.029.64285952-53
GCCGTCT6500.029.59615350-51
GTATGCC6650.029.28571546-47
ATGCCGT6750.028.49999848-49
CTAATGC7250.026.86206832-33
CGTCTTC7600.025.62552-53
GTCACTA7750.025.12903228-29
CATCTCG7700.024.98376740-41
Produced by FastQC (version 0.11.5)