FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm209RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm209RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences889468
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT48150.5413348203645325TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG7350.041.6836744-45
CGTATGC7500.040.8546-47
TATGCCG7650.040.04902348-49
CTCGTAT7550.039.95033344-45
TAATGCG7950.038.5377434-35
TCTCGTA8100.036.35802542-43
AATGCGC8700.035.48850634-35
ATCTCGT8300.035.4819342-43
TGCGCAT9150.033.22404536-37
ATGCGCA9300.033.19892536-37
GTATGCC9350.033.02139346-47
CCGTCTT9450.032.1693152-53
ATGCCGT9750.031.17948748-49
CTAATGC9900.030.94696832-33
GCCGTCT9950.030.79145850-51
GCGCATC9650.030.76424838-39
CATCTCG9600.030.67708440-41
CGCATCT9700.030.36082638-39
TGCCGTC10550.029.26540650-51
TCACTAA10900.029.19724730-31
Produced by FastQC (version 0.11.5)