FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm204RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm204RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences746781
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT26410.3536512043021984TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGCGT250.00502058957.07
TCGTATG3000.038.79166844-45
TATGCCG3100.037.5403248-49
CGTATGC3400.034.9264746-47
GCTATCG550.00257904934.5454565
CTCGTAT3450.034.42028844-45
TATCTCG3600.031.66666640-41
TCTCGTA3850.029.6103942-43
ATGCCGT4350.026.20689648-49
ATCTCGT4700.025.26595942-43
GCCGTCT4550.024.53296750-51
GTATGCC4750.024.50000246-47
CTATCTC5000.023.7540-41
CCGTCTT4950.023.03030252-53
AGCTATC5450.021.7889938-39
GCTATCT5550.020.9684738-39
TGCCGTC5700.020.00000250-51
CGTCTTC5800.019.65517452-53
GAAGCTA6400.018.92578136-37
CGCGGGT1801.232587E-418.4722211
Produced by FastQC (version 0.11.5)