FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm203RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm203RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812583
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT28670.352825495980103TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4000.038.048-49
TCGTATG3950.037.2784844-45
CGTATGC4350.034.39655346-47
CTCGTAT4700.032.34042444-45
TCTCGTA4850.031.82989942-43
TACCGTA600.003950276431.6666689
TATCTCG5100.028.8725540-41
ATCTCGT5250.028.50000242-43
GCCGTCT5600.026.7187550-51
GTATGCC5950.025.14705746-47
GCTATCT6100.024.52868838-39
AGCTATC6300.024.12698638-39
CCGTCTT6500.023.75000252-53
ATGCCGT6400.023.37890448-49
CTACCGT1050.002195671722.6190478
TGCCGTC6700.022.33208850-51
CTATCTC6750.022.16666840-41
ATCGCGC1100.00287664821.590916
CGTCTTC7750.019.91935552-53
GAGCACA16000.019.8906259
Produced by FastQC (version 0.11.5)