FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm202RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm202RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences812609
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT29400.3617976173042632TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG3450.037.8623244-45
CGTATGC3700.035.94594646-47
TATGCCG3850.034.54545648-49
CTCGTAT4100.033.01829544-45
TATCTCG4300.030.37790740-41
ATCTCGT4800.028.69791842-43
TCTCGTA4750.027.542-43
GCCGTCT5150.026.28640650-51
CCGTCTT5350.025.30373852-53
GCTATCT5200.025.12019338-39
ATGCCGT5450.024.83945348-49
GTATGCC5400.024.6296346-47
AGCTATC5900.022.94491638-39
CTATCTC6150.022.01219640-41
TGCCGTC6550.020.30534450-51
CGTCTTC6800.019.90808952-53
GAAGCTA7400.018.61486436-37
AAGCTAT7200.018.47222136-37
GCTTGAA8500.016.20588360-61
CGCGGGT1850.003142259315.4054061
Produced by FastQC (version 0.11.5)