FastQCFastQC Report
Wed 14 Aug 2019
000000000-C6PWG_l01_n01_ALm201RM.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C6PWG_l01_n01_ALm201RM.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences893136
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT31700.35492914852833163TruSeq Adapter, Index 19 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.036.14130448-49
TCGTATG4500.035.8888944-45
CGTATGC4850.033.78866246-47
CTCGTAT4950.031.66666444-45
CTACCGT1303.035584E-729.230778
TATCTCG5400.029.0277840-41
TACCGTA1056.904139E-527.1428579
TCTCGTA5850.026.79487242-43
ATCTCGT6000.026.12499842-43
ATGCCGT6400.025.60546748-49
CCGTCTT6600.024.46969852-53
TGCCGTC7050.024.2553250-51
AGCTATC6750.023.92592638-39
GCCGTCT7050.023.9184450-51
GTATGCC6850.023.57664346-47
CTATCTC7150.022.25524340-41
GCTATCT7000.022.05357238-39
TACGGAT1100.00287686921.590912
CGTCTTC8350.019.62574852-53
AAGCTAT8400.019.2261936-37
Produced by FastQC (version 0.11.5)