FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076288
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGC778750.7030785042786898No Hit
AATTCACACTGAATGTCATCTACCATGAACACAAACACACAGACAATCCC609610.5503739158822883No Hit
AATTATTATTATTTTTATTATTGTAGCAGGACGAGCCACAGACCAAACTC385050.3476345143788244No Hit
AATTCGTGTCGCTCTGATAGGAGTCCTTTTCAGTGGGTGTCTCATCCTTC351860.31766960194606714No Hit
AATTCCGGAATAGCGGGACCGGGTCCCGGGCCCTTCTCTTGTGGCAGCAG321870.29059374404132504No Hit
AATTCTGAACGTAGAACACAGGCCACACCCTCACACTGATGTTGTCTGGC151450.13673353383371756No Hit
AATTTGCTGGCACAAGTGCGTAAGGCAAATCCTCTCAACCATCAACCGTC142850.12896919978967683No Hit
AATTTTGCATCCATGTACCTGTGATACTTACATATGCATGTACACATGCA139350.12580929639966026No Hit
AATTTTGCATCCACGTACCTGTGATACTTACATATGCATGTACACATGCA132790.11988673461722918No Hit
AATTTAGAAACTGTGCGTGTGCTGTTTTCTATTTCATACTTAGAATCTGT123970.11192377807438737No Hit
AATTTGGGGGAGAAGAGACACGAGAAATGGAGACAAGACAGTGCTCTGAT122310.11042508103797952No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTGTC114750.0143.041381
AATTCAC224600.0142.93411
AATTGAC88850.0142.470461
AATTGAA119300.0142.265291
AATTGCT168350.0142.114641
AATTACA136850.0142.086231
AATTATT188050.0141.954271
AATTCGC92150.0141.931631
AATTGCC145500.0141.910641
AATTCTA98250.0141.679381
AATTCCC140050.0141.635131
AATTGAT98350.0141.535321
AATTGGT81650.0140.915481
AATTCCA205650.0140.804761
AATTAGT73500.0140.659851
AATTCTT219800.0140.580081
AATTTGG139400.0140.527251
AATTGTG111500.0140.448431
AATTAAG75600.0140.205021
AATTGAG95750.0140.078321
Produced by FastQC (version 0.11.5)