FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n02_FSddRAD4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n02_FSddRAD4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGAGACTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACA11.7241379310344827No Hit
TATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTG11.7241379310344827No Hit
TCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATG11.7241379310344827No Hit
CAATAGCAAGGCCACGACGCAATGGAGAAAGACGGAGAGCGCCAACGGCG11.7241379310344827No Hit
TAATATTCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTG11.7241379310344827No Hit
AGTATTTTTGTGTGCCTGAGTATGGTACAGCTAATGGCCGTCTTCATTTC11.7241379310344827No Hit
AGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACATACCAAAG11.7241379310344827No Hit
CATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGATTCTGAACA11.7241379310344827No Hit
CCCTGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTAATCGTGCC11.7241379310344827No Hit
GCGAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTT11.7241379310344827No Hit
AACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAGCTGTTCAGAAT11.7241379310344827No Hit
AACTGGCCTAACGACGTTTGGTCAGTTCCATCAACATCATAGCCAGATGC11.7241379310344827No Hit
GACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATCTCGGCAAT11.7241379310344827No Hit
ACCCTAAGGCCACGTATTTTGCAAGCTATTTAACTGGCGGCGATTGCGTA11.7241379310344827No Hit
CAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGATGGTTGGTTTAT11.7241379310344827No Hit
CGGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTC11.7241379310344827No Hit
TGCTTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTC11.7241379310344827No Hit
GTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACG11.7241379310344827No Hit
GTTTACTCTTGCGCTTGTTCGTTTTCCGCCTACTGCGACTAAAGAGATTC11.7241379310344827No Hit
AGATTTGTCATTGTGAGCATTTTCATCCCGAAGTTGCGGCTCATTCTGAT11.7241379310344827No Hit
ACCATTATCGAACTCAACGCCCTGCATACGAAAAGACAGAATCTCTTCCA11.7241379310344827No Hit
ATACTGTTTTCCGTAAATTCAGCGCCTTCCATGATGAGACAGGCCGTTTG11.7241379310344827No Hit
AGCGTATCGAGGCTCTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACT11.7241379310344827No Hit
AATTGTTGACCACCTACATACCAAAGACGAGCGCCTTTACGCTTGCCTTT11.7241379310344827No Hit
ATCCGTGACGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAAG11.7241379310344827No Hit
TGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCAACTAACG11.7241379310344827No Hit
GTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAGGAAGTGTCCGC11.7241379310344827No Hit
ATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGGTTG11.7241379310344827No Hit
CCGCCTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTA11.7241379310344827No Hit
CCATAACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCGGTACG11.7241379310344827No Hit
ACTGTAGCCGACGTTTTGGCGGCGCAACCTGTGACGACAAATCTGCTCAA11.7241379310344827No Hit
CCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTC11.7241379310344827No Hit
ATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAAAGTAAAAAT11.7241379310344827No Hit
GGCGGCAAGTTGCCATACAAAACAGGGTCGCCAGCAATATCGGAATAAGT11.7241379310344827No Hit
CACACTCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGC11.7241379310344827No Hit
TGGTTTTTAGAGAACGAGAAGACGGTTACGCAGTTTTGCCGCAAGCTGGC11.7241379310344827No Hit
TCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCATCAGCACCAGCA11.7241379310344827No Hit
CTTCTGGTGATTTGCAAGAACGCGTACTTATTCGCCACCATGATTATGAC11.7241379310344827No Hit
AGCTTGGTAAGTTGGATTAAGCACTCCGTGGACAGATTTGTCATTGTGAG11.7241379310344827No Hit
TACTAAAGGCAAGCGTAAAGGCGCTCGTCTTTGGTATGTAGGTGGTCAAC11.7241379310344827No Hit
AGCCTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTT11.7241379310344827No Hit
AAAGACGAGCGCCTTTACGCTTGCCTTTAGTACCTCGCAACGGCTGCGGA11.7241379310344827No Hit
TATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACG11.7241379310344827No Hit
AGCAACTTATCAGAAACGGCAGAAGTGCCAGCCTGCAACGTACCTTCAAG11.7241379310344827No Hit
CGATGCTGTTCAACCACTAATAGGTAAGAAATCATGAGTCAAGTTACTGA11.7241379310344827No Hit
AATCGAAGTGGACTGCTGGCGGAAAATGAGAAAATTCGACCTATCCTTGC11.7241379310344827No Hit
AGGATAGGTCGAATTTTCTCATTTTCCGCCAGCAGTCCACTTCGATTTAA11.7241379310344827No Hit
CTATCCTTGCGCAGCTCGAGAAGCTCTTACTTTGCGACCTTTCGCCATCA11.7241379310344827No Hit
CAAGTTACTGAACAATCCGTACGTTTCCAGACCGCTTTGGCCTCTATTAA11.7241379310344827No Hit
TATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACTTGCCGCCGCG11.7241379310344827No Hit
ATTTGTTTCAGGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCG11.7241379310344827No Hit
TTGCTGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCT11.7241379310344827No Hit
GACAGCTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTT11.7241379310344827No Hit
GGCCTCCAGCAATCTTGAACACTCATCCTTAATACCTTTCTTTTTGGGGT11.7241379310344827No Hit
CCCAATGCTTTGCGTGACTATTTTCGTGATATTGGTCGTATGGTTCTTGC11.7241379310344827No Hit
ATAGCACCAGAAACAAAATTAGGGACGGCCTCATCAGGGTTTGGAACATT11.7241379310344827No Hit
TCTACGCGATTTCATAGTGGAGGCCTCCAGCAATCTTGAACACTCATCCT11.7241379310344827No Hit
TCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTTCTC11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGT50.0145.07
CGTTTAT50.0145.04
ACGTTTA50.0145.03
CACGTTT50.0145.02
ATGGTGA50.0145.09
GTTTATG50.0145.05
TCACGTT50.0145.01
TTTATGG50.0145.06
TTTGTTT50.0145.0145
TATGGTG50.0145.08
Produced by FastQC (version 0.11.5)