FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n02_FSddRAD1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n02_FSddRAD1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGATTGCGATAAACGGTCACATTAAATTTAACCTGACTATTCCACTGCA23.9215686274509802No Hit
TATCAAGTTGGGGGAGCACATTGTAGCATTGTGCCAATTCATCCATTAAC11.9607843137254901No Hit
TGTCCCTCATCGTCACGTTTATGGTGAACAGTGGATTAAGTTCATGAAGG11.9607843137254901No Hit
TGCCGAGATGCAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGG11.9607843137254901No Hit
TCTGGTTGAACGGCGTCGCGTCGTAACCCAGCTTGGTAAGTTGGATTAAG11.9607843137254901No Hit
TTTTTGGGGTAATTATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGC11.9607843137254901No Hit
CCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTT11.9607843137254901No Hit
TGGATGAGGAGAAGTGGCTTAATATGCTTGGCACGTTCGTCAAGGACTGG11.9607843137254901No Hit
TTCTTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTATGT11.9607843137254901No Hit
GGGTTATTTGAATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTG11.9607843137254901No Hit
AATCTCACGAAAATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCT11.9607843137254901No Hit
CATAAAACGCCTCTAATCGGTCGTCAGCCAACGTGAGAGTGTCAAAAACG11.9607843137254901No Hit
TCAGTATTTTACCAATGACCAAATCAAAGAAATGACTCGCAAGGTTAGTG11.9607843137254901No Hit
CTTCTTCGGCACCTGTTTTACAGACACCTAAAGCTACATCGTCAACGTTA11.9607843137254901No Hit
GAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCACCAAGTCCAACCAAA11.9607843137254901No Hit
GTGATGAGTCTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAA11.9607843137254901No Hit
TACTAAAATGCAACTGGACAATCAGAAAGAGATTGCCGAGATGCAAAATG11.9607843137254901No Hit
CAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCA11.9607843137254901No Hit
ATGGTTCGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTT11.9607843137254901No Hit
TTATTGGTATCAGGGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATA11.9607843137254901No Hit
TGGTGTTTTCCATAATAGACGCAACGCGAGCAGTAGACTCCTTCTGTTGA11.9607843137254901No Hit
AGCAAGATAATCACGAGTATCCTTTCCTTTATCAGCGGCAGACTTGCCAC11.9607843137254901No Hit
ACGTTATATTTTGATAGTTTGACGGTTAATGCTGGTAATGGTGGTTTTCT11.9607843137254901No Hit
ACATAATTTATCCTCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTGT11.9607843137254901No Hit
GAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAGGGACG11.9607843137254901No Hit
ATCCTTTGGATGGTCGCCATGATGGTGGTTATTATACCGTCAAGGACTGT11.9607843137254901No Hit
GTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCTATTAGTGGTT11.9607843137254901No Hit
TCAAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCC11.9607843137254901No Hit
AAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCT11.9607843137254901No Hit
GGAAAGACGGTAAAGCTGATGGTATTGGCTCTAATTTGTCTAGGAAATAA11.9607843137254901No Hit
AGAAAGAGTAGAAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGA11.9607843137254901No Hit
TGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATT11.9607843137254901No Hit
TAAGCAGAAAACCTACCGCGCTTCGCTTGGTCAACCCCTCAGCGGCAAAA11.9607843137254901No Hit
AAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGTAGGCGG11.9607843137254901No Hit
TTCCAGTCCGTTCAGTTGTTGCAGTGGAATAGTCAGGTTAAATTTAATGT11.9607843137254901No Hit
AAGCTGTTGCCGATACTTGGAACAATTTCTGGAAAGACGGTAAAGCTGAT11.9607843137254901No Hit
GCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTTCAATATCTGGTTGAAC11.9607843137254901No Hit
AAAGGATAAACATCATAGGCAGTCGGGAGGGTAGTCGGAACCGAAGAAGA11.9607843137254901No Hit
GGTGGTCTATAGTGTTATTAATATCAAGTTGGGGGAGCACATTGTAGCAT11.9607843137254901No Hit
AACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGACGAATCACCA11.9607843137254901No Hit
ACAGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCT11.9607843137254901No Hit
CCATCTCAAAAACATTTGGACTGCTCCGCTTCCTCCTGAGACTGAGCTTT11.9607843137254901No Hit
GAGCTTAATGCTTGGGAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTAT11.9607843137254901No Hit
GATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCT11.9607843137254901No Hit
TCCTTGACGGTATAATAACCACCATCATGGCGACCATCCAAAGGATAAAC11.9607843137254901No Hit
CCATTCTTTAGCTCCTAGACCTTTAGCAGCAAGGTCCATATCTGACTTTT11.9607843137254901No Hit
ATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCATCA11.9607843137254901No Hit
GCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGA11.9607843137254901No Hit
AATGTTTATAGGTCTGTTGAACACGACCAGAAAACTGGCCTAACGACGTT11.9607843137254901No Hit
CGTGGATGCCTGACCGTACCGAGGCTAACCCTAATGAGCTTAATCAAGAT11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG50.0145.02
TCAAGTT50.0145.0145
GGGTAAT50.0145.07
TTTTTGG50.0145.01
TGGGGTA50.0145.05
TTGGGGT50.0145.04
TTTGGGG50.0145.03
GGGGTAA50.0145.06
GTAATTA50.0145.09
GGTAATT50.0145.08
Produced by FastQC (version 0.11.5)