FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11076288
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCATCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA369210.3333336944651493No Hit
GCATGCATGCACACACACACACCATCACATACGCAGTCAATCCGACATCA258570.23344463415902514No Hit
GCATGCATGCCCTTGAGTCGTTTGTGCGGCGGACTCTGCCTGGCCCTGCT138430.12497869322285587No Hit
TCGATCATGCACACACACACACCATCACATACGCAGTCAATCCGACATCA130340.11767480224421756No Hit
CAACCCATGCACACACACACACCATCACATACGCAGTCAATCCGACATCA126610.11430724805999988No Hit
TCGATCATGCTAACTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAA114150.10305799199154084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCATGC2958700.0142.907354
GCATGCC1020900.0142.613865
GCATGCT688150.0142.471485
GCATGCG351650.0140.464235
CAACCCA1715600.0140.279631
AACCCAT1719400.0140.04552
TCGATCA1314350.0139.925251
CGATCAT1318200.0139.461582
CGATCCA658850.0137.89141
ACCCATG1761400.0136.677373
ATCATGC2586750.0135.700494
GATCCAT670400.0134.899322
TTGCATG775450.0134.248173
CCCATGC1792700.0134.16164
ATGCATG2402650.0132.899833
CATGCTA311400.0132.381186
TGCATCA1272250.0132.229521
GCATCAT1278800.0131.478532
CATGCGA185550.0130.308556
GTTGCAT799300.0130.233322
Produced by FastQC (version 0.11.5)