FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n01_FSddRAD4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n01_FSddRAD4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCTCTTTAGTCGCAGTAGGCGGAAAACGAACAAGCGCAAGAGTAAACA23.4482758620689653No Hit
ATTTTTCATGGTATTGATAAAGCTGTTGCCGATACTTGGAACAATTTCTG11.7241379310344827No Hit
AGTCGGGAGAGGAGTGGCATTAACACCATCCTTCATGAACTTAATCCACT11.7241379310344827No Hit
CAAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACG11.7241379310344827No Hit
CTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGC11.7241379310344827No Hit
AGTAGTAATTCCTGCTTTATCAAGATAATTTTTCGACTCATCAGAAATAT11.7241379310344827No Hit
TCGGCGTGTGAATCATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACG11.7241379310344827No Hit
GTCAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGA11.7241379310344827No Hit
ACCGCATCAAGCTCTTGGAAGAGATTCTGTCTTTTCGTATGCAGGGCGTT11.7241379310344827No Hit
CCATGATGGTGGTTATTATACCGTCAAGGACTGTGTGACTATTGACGTCC11.7241379310344827No Hit
ATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCATGATGTTATT11.7241379310344827No Hit
GGTTGTGGCCTGTTGATGCTAAAGGTGAGCCGCTTAAAGCTACCAGTTAT11.7241379310344827No Hit
GTCCGCAGCCGTTGCGAGGTACTAAAGGCAAGCGTAAAGGCGCTCGTCTT11.7241379310344827No Hit
TATACTCATCGCGAATATCCTTAAGAGGGCGTTCAGCAGCCAGCTTGCGG11.7241379310344827No Hit
AATATCTATAACAACTATTTTAAAGCGCCGTGGATGCCTGACCGTACCGA11.7241379310344827No Hit
AAGCTGGGTTACGACGCGACGCCGTTCAACCAGATATTGAAGCAGAACGC11.7241379310344827No Hit
TAACCACCATCATGGCGACCATCCAAAGGATAAACATCATAGGCAGTCGG11.7241379310344827No Hit
ACTGGACCCTCAGAAAGAGATTGCCGAGATGCAAAATGAGACTCAAAAAG11.7241379310344827No Hit
GTGCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGG11.7241379310344827No Hit
ACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAGGTTATTAAAGA11.7241379310344827No Hit
TTAAGAGGGCGTTCAGCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTT11.7241379310344827No Hit
CAAACTTTGTTACTCGTCAGAAAATCGAAATCATCTTCGGTTAAATCCAA11.7241379310344827No Hit
TAAACGGTCACATTAAATTTAACCTGACTATTCCACTGCAACAACTGAAC11.7241379310344827No Hit
CGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGGCCACGTATTTT11.7241379310344827No Hit
CTGGTATTAAATCTGCCATTCAAGGCTCTAATGTTCCTAACCCTGATGAG11.7241379310344827No Hit
TCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTATCAGGGTTA11.7241379310344827No Hit
TAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATG11.7241379310344827No Hit
GTTCATCCCGTCAACATTCAAACGGCCTGTCTCATCATGGAAGGCGCTGA11.7241379310344827No Hit
TTTCGTATGCAGGGCGTTGAGTTCGATAATGGTGATATGTATGTTGACGG11.7241379310344827No Hit
TCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAACAATTCAGCGGC11.7241379310344827No Hit
GAGCGTGCTGGTGCTGATGCTTCCTCTGCTGGTATGGTTGACGCCGGATT11.7241379310344827No Hit
TTAGGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAG11.7241379310344827No Hit
CTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAA11.7241379310344827No Hit
CGTCGCTGCGTTGAGGCTTGCGTTTATGGTACGCTGGACTTTGTAGGATA11.7241379310344827No Hit
CGCTGATTATTTTGACTTTGAGCGTATCGAGGCTCTTAAACCTGCTATTG11.7241379310344827No Hit
TAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAAGAGGTTTTA11.7241379310344827No Hit
TGGTACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGA11.7241379310344827No Hit
AAACTGGCGCCGAGCGTATGCCGCATGACCTTTCCCATCTTGGCTTCCTT11.7241379310344827No Hit
AGTCCTTGACGAACGTGCCAAGCATATTAAGCCACTTCTCCTCATCCAAC11.7241379310344827No Hit
CTATGAAATCGCGTAGAGGCTTTGCTATTCAGCGTTTGATGAATGCAATG11.7241379310344827No Hit
GCACTTTATGCGGACACTTCCTACAGGTAGCGTTGACCCTAATTTTGGTC11.7241379310344827No Hit
TTCTCACTTCTGTTACTCCAGCTTCTTCGGCACCTGTTTTACAGACACCT11.7241379310344827No Hit
AAGAATGGAACAACTCACTAAAAACCAAGCTGTCGCTACTTCCCAAGAAG11.7241379310344827No Hit
CTTGGTTTTTAGTGAGTTGTTCCATTCTTTAGCTCCTAGACCTTTAGCAG11.7241379310344827No Hit
CGTGCAGAAGGAGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCT11.7241379310344827No Hit
TGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTGTATGGCAACT11.7241379310344827No Hit
GATACCCTCGCTTTCCTGCTCCTGTTGAGTTTATTGCTGCCGTCATTGCT11.7241379310344827No Hit
GTCTTATTACCATTTCAACTACTCCGGTTATCGCTGGCGACTCCTTCGAG11.7241379310344827No Hit
TTCTGCTCTTGCTGGTGGCGCCATGTCTAAATTGTTTGGAGGCGGTCAAA11.7241379310344827No Hit
GTTTGAATTATGGCGAGAAATAAAAGTCTGAAACATGATTAAACTCCTAA11.7241379310344827No Hit
AGAAGTCGTCATTTGGCGAGAAAGCTCAGTCTCAGGAGGAAGCGGAGCAG11.7241379310344827No Hit
TCAATCTTTTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATT11.7241379310344827No Hit
AGGCTCATGCTGATGGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCT11.7241379310344827No Hit
GGTGTCTGTAAAACAGGTGCCGAAGAAGCTGGAGTAACAGAAGTGAGAAC11.7241379310344827No Hit
ATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGAATGAGCT11.7241379310344827No Hit
ACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCTGCTT11.7241379310344827No Hit
CCAGCACGCTCCCAAGCATTAAGCTCAGGAAATGCAGCAGCAAGATAATC11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCATT50.0145.07
GTCATTT50.0145.08
AGAAGTC50.0145.01
GTCGTCA50.0145.05
GAAGTCG50.0145.02
CGGCGCT50.0145.0145
AGTCGTC50.0145.04
TCGTCAT50.0145.06
TCATTTG50.0145.09
AAGTCGT50.0145.03
Produced by FastQC (version 0.11.5)