FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n01_FSddRAD2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n01_FSddRAD2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAACTGGTAGCTTTAAGCGGCTCACCTTTAGCATCAACAGGCCACA21.7241379310344827No Hit
AGAAAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGAAT21.7241379310344827No Hit
GGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGG10.8620689655172413No Hit
AATCGCGAGTGGTCGGCAGATTGCGATAAACGGTCACATTAAATTTAACC10.8620689655172413No Hit
AGTGCCAGCCTGCAACGTACCTTCAAGAAGTCCTTTACCAGCTTTAGCCA10.8620689655172413No Hit
GCAATACCGCCAGCAATAGCACCAAACATAAATCACCTCACTTAAGTGGC10.8620689655172413No Hit
AGCCAGATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCA10.8620689655172413No Hit
GAATGGGAAGCCTTCAAGAAGGTGATAAGCAGGAGAAACATACGAAGGCG10.8620689655172413No Hit
TAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAATCTCTTTAGTCGCAGT10.8620689655172413No Hit
AAATCGAAATCATCTTCGGTTAAATCCAAAACGGCAGAAGCCTGAATGAG10.8620689655172413No Hit
CAGCGGCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAA10.8620689655172413No Hit
TAATAAGAACGAACCATAAAAAAGCCTCCAAGATTTGGAGGCATGAAAAC10.8620689655172413No Hit
AAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTA10.8620689655172413No Hit
GACGGCAGCAATAAACTCAACAGGAGCAGGAAAGCGAGGGTATCCTACAA10.8620689655172413No Hit
TCTATTATGGAAAACACCAATCTTTCCAAGCAACAGCAGGTTTACGAGAT10.8620689655172413No Hit
AAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTACACG10.8620689655172413No Hit
GAATGCAATGAAGAAAACCACCATTACCAGCATTAACCGTCAAACTATCA10.8620689655172413No Hit
TTGAGAATCAAAAAGAGCTTACTAAAATGCAACTGGACAATCAGAAAGAG10.8620689655172413No Hit
TTAAAATGTCAACAAGAGAATCTCTACCATGAACAAAATGTGACTCATAT10.8620689655172413No Hit
TTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCCATATAACTGGT10.8620689655172413No Hit
CGACATTAGAAATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATAC10.8620689655172413No Hit
TTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGCAGAAGT10.8620689655172413No Hit
AAAGCGTCCTGCGTGTAGCGAACTGCGATGGGCATACTGTAACCATAAGG10.8620689655172413No Hit
TCAACCATACCAGCAGAGGAAGCATCAGCACCAGCACGCTCCCAAGCATT10.8620689655172413No Hit
CTGTGACGACAAATCTGCTCAAATTTATGCGCGCTTCGATAAAAATGATT10.8620689655172413No Hit
TCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAATAATCAGC10.8620689655172413No Hit
GCTAATTTGCATACTGACCAAGAACGTGATTACTTCATGCAGCGTTACCA10.8620689655172413No Hit
TTGAACAGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAA10.8620689655172413No Hit
GCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGCTGCGCAAGGATAGGTCGA10.8620689655172413No Hit
CCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCATAA10.8620689655172413No Hit
GAGTGGCATTAACACCATCCTTCATGAACTTAATCCACTGTTCACCATAA10.8620689655172413No Hit
GAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATCCATCTGCTTA10.8620689655172413No Hit
AAGTCGCCGACTGAATGCCAGCAATCTCTTTTTGAGTCTCATTTTGCATC10.8620689655172413No Hit
TTATCACGAAGTCATGATTGAATCGCGAGTGGTCGGCAGATTGCGATAAA10.8620689655172413No Hit
CCGACTGCCTATGATGTTTATCCTTTGGATGGTCGCCATGATGGTGGTTA10.8620689655172413No Hit
ATAGATATTCAAATAACCCTGAAACAAATGCTTAGGGATTTTATTGGTAT10.8620689655172413No Hit
TAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTG10.8620689655172413No Hit
TGAATCAGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGT10.8620689655172413No Hit
GCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAAAAAGCG10.8620689655172413No Hit
TTTAGACATTACATCACTCCTTCTGCACGTAATTTTTGACGCACGTTTTC10.8620689655172413No Hit
CATTATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTG10.8620689655172413No Hit
GCTTTGCTATTCAGCGTTTGATGAATGCAATGCGACAGGCTCATGCTGAT10.8620689655172413No Hit
CTGACTATTCCACTGCAACAACTGAACGGACTGGAAACACTGGTCATAAT10.8620689655172413No Hit
TTTGGTCATTGGTAAAATACTGACCAGCCGTTTGAGCTTGAGTAAGCATT10.8620689655172413No Hit
GCAGCCAGCTTGCGGCAAAACTGCGTAACCGTCTTCTCGTTCTCTAAAAA10.8620689655172413No Hit
AAGAAATCATGAGTCAAGTTACTGAACAATCCGTACGTTTCCAGACCGCT10.8620689655172413No Hit
TGCTAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGC10.8620689655172413No Hit
AAATAGTCACGCGAAGCATTGGGATTATCATAAAACGCCTCTAATCGGTC10.8620689655172413No Hit
CCATAAAAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAATTGGGAG10.8620689655172413No Hit
CAGAATCGTTAGTTGATGGCGAAAGGTCGCAAAGTAAGAGCTTCTCGAGC10.8620689655172413No Hit
CTCTTTTTGATTCTCAAATCCGGCGTCAACCATACCAGCAGAGGAAGCAT10.8620689655172413No Hit
CTGACGGTTATTTCCTAGACAAATTAGAGCCAATACCATCAGCTTTACCG10.8620689655172413No Hit
CATAATCATGGTGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAAGGC10.8620689655172413No Hit
GTTATCGGTAGCAAGCACATCACCTTGAATGCCACCGGAGGCGGCTTTTT10.8620689655172413No Hit
GAATCATTAGCCTTGCGACCCTCGGCAGCAAGAACCATACGACCAATATC10.8620689655172413No Hit
GAATGAGCCGCAACTTCGGGATGAAAATGCTCACAATGACAAATCTGTCC10.8620689655172413No Hit
CAGCAAGAACCATACGACCAATATCACGAAAATAGTCACGCAAAGCATTG10.8620689655172413No Hit
TATGGTGAACAGTGGATTAAGTTCATGAAGGATGGTGTTAATGCCACTCC10.8620689655172413No Hit
GGAAACCATAACGAGCATCATCTTGATTAAGCTCATTAGGGTTAGCCTCG10.8620689655172413No Hit
CAGAAACAAAACTAGGGACGGCCTCATCAGGGTTAGGAACATTAGAGCCT10.8620689655172413No Hit
TCTGGGCATCTGGCTATGATGTTGATGGAACTGACCAAACGTCGTTAGGC10.8620689655172413No Hit
TTCTGGCGCTCGCCCTGGTCGTCAGAAGAAGATGAGAGGTACTAAAGGCA10.8620689655172413No Hit
GAGCACGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAA10.8620689655172413No Hit
AAAATGAGACTCAAAAAGAGATTGCTGGCATTCAGTCGGCGACTTCACGC10.8620689655172413No Hit
TTGCAAGCTATTTAACTGGCGGCGATTGCGTACCCGACGACCAAAATTAG10.8620689655172413No Hit
TAAAGGTCTAGGAGCTAAAGAATGGAACAACTCACTAAAAACCAAGCTGT10.8620689655172413No Hit
TCACCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTT10.8620689655172413No Hit
AATTTAGACATGGCGCCACCAGCAAGAGCAGAAGCAATACCGCCAGCAAT10.8620689655172413No Hit
GTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAATGT10.8620689655172413No Hit
GCCTCTATTAAGCTCATTCAGGCTTCTGCCGTTTTGGATTTAACCGAAGA10.8620689655172413No Hit
AATGCTGGTACTGGTGGTTTTCTTCATTGCATTCAGATGGATACATCTGT10.8620689655172413No Hit
AGTCACACAGTCCTTGACGGTATAATAACCACCATCATGGCGACCATCCA10.8620689655172413No Hit
CGCTACTTCCCAAGAAGCTGTTCAGAATCAGAATGAGCCGCAACTTCGGG10.8620689655172413No Hit
CAAAAAGAGAGATGAGATTGAGGCTGGGAAAAGTTACTGTAGCCGACGTT10.8620689655172413No Hit
GGTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACC10.8620689655172413No Hit
AAGAGCCATACCGCTGATTCTGCGTTTGCTGATGAACTAAGTCAACCTCA10.8620689655172413No Hit
CCATATCTGACTTTTTGTTAACGTATTTAGCCACATAGAAACCAACAGCC10.8620689655172413No Hit
TGAGGTTGACTTAGTTCATCAGCAAACGCAGAATCAGCGGTATGGCTCTT10.8620689655172413No Hit
AAGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACATACCAAA10.8620689655172413No Hit
CGTGATATTGGTCGTATGGTTCTTGCTACCGAGGGTCGCAAGGCTAATGA10.8620689655172413No Hit
GTCTGGAAACGTACGGATTGTTCAGTAACTTGACTCATGATTTCTTACCT10.8620689655172413No Hit
TTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGTGTTAAC10.8620689655172413No Hit
AGCATCGGACTCAGATAGTAATCCACGCTCTTTTAAAATGTCAACAAGAG10.8620689655172413No Hit
CATTCAGTCGGCGACTTCACGCCAGAATACGAAAGACCAGGTATATGCAC10.8620689655172413No Hit
CCAGCAATATCGGTATAAGTCAAAGCACCTTTAGCGTTAAGGTACTGAAT10.8620689655172413No Hit
AAGGCGCATAACGATACCACTGACCCTCAGCAATCTTAAACTTCTTAGAC10.8620689655172413No Hit
GCGGCGATTGCGTACCCGACGACCAAAATTAGGGTCAACGCTACCTGTAG10.8620689655172413No Hit
ATAGTCACGCAAAGCATTGGGATTATCATAAAACGCCTCTAATCGGTCGT10.8620689655172413No Hit
CAGTAAGAACGTCAGTGTTTCCTGCGCGTACACGCAAGGTAAACGCGAAC10.8620689655172413No Hit
AAAGTAAAAATGTCTACAGTAGAGTCAATAGCAAGGCCACGACGCAATGG10.8620689655172413No Hit
TGAGGGTCAGTGGTATCGTTATGCGCCTTCGTATGTTTCTCCTGCTTATC10.8620689655172413No Hit
AGGTTTAAGAGCCTCGATACGCTCAAAGTCAAAATAATCAGCGTGACATT10.8620689655172413No Hit
TGCAAAATACGTGGCCTTATGGTTACAGTATGCCCATCGCAGTTCGCTAC10.8620689655172413No Hit
TTGCCATACAAAACAGGGTCGCCAGCAATATCGGTATAAGTCAAAGCACC10.8620689655172413No Hit
TCCACGCTCTTTTAAAATGTCAACAAGAGAATCTCTACCATGAACAAAAT10.8620689655172413No Hit
CGAGAGCGGTCAGTAGCAATCCAAACTTTGTTACTCGTCAGAAAATCGAA10.8620689655172413No Hit
TCAGGGTTAATCGTGCCAAGAAAAGCGGCATGGTCAATATAACCAGTAGT10.8620689655172413No Hit
CCAGAACGGAAAACATCCTTCATAGAAATTTCACGCGGCGGCAAGTTGCC10.8620689655172413No Hit
CGTTTTCCGCCTACTGCGACTAAAGAGATTCAGTACCTTAACGCTAAAGG10.8620689655172413No Hit
ATGAACTTAATCCACTGTTCACCATAAACGTGACGATGAGGGACATAAAA10.8620689655172413No Hit
TAAGCGGCTCACCTTTAGCATCAACAGGCCACAACCAACCAGAACGTGAA10.8620689655172413No Hit
GCGTCATAAGAGGTTTTACCTCCAAATGAAGAAATAACATCATGGTAACG10.8620689655172413No Hit
CAGAAATTGTTCCAAGTATCGGCAATAGCTTTATCAATACCATGAAAAAT10.8620689655172413No Hit
TGGCGAATAAGTACGCGTTCTTGCAAATCACCAGAAGGCGGTTCCTGAAT10.8620689655172413No Hit
AGTGATGTAATGTCTAAAGGTAAAAAACGTTCTGGCGCTCGCCCTGGTCG10.8620689655172413No Hit
TGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAACACTGACGTTC10.8620689655172413No Hit
CCCTGCATACGAAAAGACAGAATCTCTTCCAAGAGCTTGATGCGGTTATC10.8620689655172413No Hit
GAGGGTAGTCGGAACCGAAGAAGACTCAAAGCGAACCAAACAGGCAAAAA10.8620689655172413No Hit
CACACGCCGACTGCTATCAGTATTTTTGTGTGCCTGAGTATGGTACAGCT10.8620689655172413No Hit
TGGAAACCCTGGTCATAATCATGGTGGCGAATAAGTACGCGTTCTTGCAA10.8620689655172413No Hit
TTCAAGAAGTCCTTTACCAGCTTTAGCCATAGCACCAGAAACAAAACTAG10.8620689655172413No Hit
AAATGCCACAAGCCTCAATAGCAGGTTTAAGAGCCTCGATACGCTCAAAG10.8620689655172413No Hit
AGCCACAACCAACCAGAACGTGAAAAAGCGTCCTGCGTGTAGCGAACTGC10.8620689655172413No Hit
TTTAATTTTTGCCGCTGAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTC10.8620689655172413No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers