FastQCFastQC Report
Tue 6 Aug 2019
000000000-C5HTY_l01_n01_FSddRAD1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-C5HTY_l01_n01_FSddRAD1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTATGCCCATCGCAGTTCGCTACACGCAGGACGCTTTTTCACGTTCTGG23.9215686274509802No Hit
AGCCAACGTGAGAGTGTCAAAAACGATAAACCAACCATCAGCATGAGCCT11.9607843137254901No Hit
TGCCGTCATTGCTTATTATGTTCATCCCGTCAACATTCAAACGGCCTGTC11.9607843137254901No Hit
GTAACCGTCTTCTCGTTCTCTAAAAACCATTTTTCGTCCCCTTCGGGGCG11.9607843137254901No Hit
GAGTCATTTCTTTGATTTGGTCATTGGTAAAATACTGACCAGCCGTTTGA11.9607843137254901No Hit
CGCCGGGCAATAATGTTTATGTTGGTTTCATGGTTTGGTCTAACTTTACC11.9607843137254901No Hit
AATATCCTTTGCAGTAGCGCCAATATGAGAAGAGCCATACCGCTGATTCT11.9607843137254901No Hit
TCCGTTCTGGTGATTCGTCTAAGAAGTTTAAGATTGCTGAGGGTCAGTGG11.9607843137254901No Hit
GGTTGGTTTATCGTTTTTGACACTCTCACGTTGGCTGACGACCGATTAGA11.9607843137254901No Hit
TCAAGTAAGGGGCCGAAGCCCCTGCAATTAAAATTGTTGACCACCTACAT11.9607843137254901No Hit
AGCGGCGTTGACAGATGTATCCATCTGAATGCAATGAAGAAAACCACCAT11.9607843137254901No Hit
GCAGACTTGCCACCAAGTCCAACCAAATCAAGCAACTTATCAGAAACGGC11.9607843137254901No Hit
GATAAAGCAGGAATTACTACTGCTTGTTTACGAATTAAATCGAAGTGGAC11.9607843137254901No Hit
TTTGTATCTGTTACTGAGAAGTTAATGGATGAATTGGCACAATGCTACAA11.9607843137254901No Hit
TACAGCTAATGGCCGTCTTCATTTCCATGCGGTGCACTTTATGCGGACAC11.9607843137254901No Hit
ATGAATTGGCACAATGCTACAATGTGCTCCCCCAACTTGATATTAATAAC11.9607843137254901No Hit
CTGAGCTTTCTCGCCAAATGACGACTTCTACCACATCTATTGACATTATG11.9607843137254901No Hit
TACCGAGGCTAACCCTAATGAGCTTAATCAAGATGATGCTCGTTATGGTT11.9607843137254901No Hit
GTAATCACGTTCTTGGTCAGTATGCAAATTAGCATAAGCAGCTTGCAGAC11.9607843137254901No Hit
TTAGAGCCTTGAATGGCAGATTTAATACCAGCATCACCCATGCCTACAGT11.9607843137254901No Hit
GCTTGTTCGTTTTCCGCCTAATGCGACTAAAGAGATTCAGTACCTTAACG11.9607843137254901No Hit
AAGCGAGGGTATCCTACAAAGTCCAGCGTACCATAAACGCAAGCCTCAAC11.9607843137254901No Hit
CACGGCGCTTTAAAATAGTTGTTATAGATATTCAAATAACCCTGAAACAA11.9607843137254901No Hit
CCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCCAATGCT11.9607843137254901No Hit
CGTTCTTATTACCCTTCTGAATGTCACGCTGATTATTTTGACTTTGAGCG11.9607843137254901No Hit
ATGTTATTTCTTCATTTGGAGGTAAAACCTCTTATGACGCTGACAACCGT11.9607843137254901No Hit
CAGGACGCTTTTTCACGTTCTGGTTGGTTGTGGCCTGTTGATGCTAAAGG11.9607843137254901No Hit
GGCTACAGTAACTTTTCCCAGCCTCAATCTCATCTCTCTTTTTGCGTTCT11.9607843137254901No Hit
TCAACATACATATCACCATTATCGAACTCAACGCCCTGCATACGAAAAGA11.9607843137254901No Hit
GAGGGGTTGACCAAGCGAAGCGCGGTAGGTTTTCTGCTTAGGAGTTTAAT11.9607843137254901No Hit
GAAACCAACATAAACATTATTGCCCGGCGTACGGGGAAGGACGTCAATAG11.9607843137254901No Hit
CTTAAACCTGCTATTGAGGCTTGTGGCATTTCTACTCTTTCTCAATCCCC11.9607843137254901No Hit
GAAGGTACGTTGCAGGCTGGCACTTCTGCCGTTTCTGATAAGTTGCTTGA11.9607843137254901No Hit
ACAAGCGCCAGAGTAAACATAGTGCCATGCTCAGGAACAAAGAAACGCGG11.9607843137254901No Hit
GAACCATAAAAAAGCCTCCAAGATTTGGAGGCATGAAAACATACAATTGG11.9607843137254901No Hit
TATTGCCCGGCGTACGGGGAAGGACGTCAATAGTCACACAGTCCTTGACG11.9607843137254901No Hit
AGGTTATTAAAGAGATTATTTGTCTCCAGCCACTTAAGTGAGGTGATTTA11.9607843137254901No Hit
ATGCCCAGAGATTAGAGCGCATGACAAGTAAAGGACGGTTGTCAGCGTCA11.9607843137254901No Hit
AAACGCAGAATCAGCGGTATGGCTCTTCTCATATTGGCGCTACTGCAAAG11.9607843137254901No Hit
CACCAGAAGCAGAATCAGTGACGACATTAGAAATATCCTTTGCAGTAGCG11.9607843137254901No Hit
GGTCTAACTTTACCGCTACTAAATGCCGCGGATTGGTTTCGCTGAATCAG11.9607843137254901No Hit
ACGCTAAAGGTGCTTTGACTTATACCGATATTGCTGGCGACCCTGTTTTG11.9607843137254901No Hit
AGCATTGTGCCAATTCATCCATTAACTTCTCAGTAACAGATACAAACTCA11.9607843137254901No Hit
ACACCCTCCCAATTGTATGTTTTCATGCCTCCAAATCTTGGAGGCTTTTT11.9607843137254901No Hit
GACTTTTATTTCTCGCCATAATTCAAACTTTTTTTCTGATAAGCTGGTTC11.9607843137254901No Hit
CTGTTGAACACGACCAGAAAACTGGCCTAACGACGTTTGGTCAGTTCCAT11.9607843137254901No Hit
ATTTATGTTTGGTGCTATTGCTGGCGGTATTGCTTCTGCTCTTGCTGGTG11.9607843137254901No Hit
TCCATAAGCAGATGGATAACCGCATCAAGCTCTTGGAAGAGATTCTGCCT11.9607843137254901No Hit
CCCCTGCAATTACAATTGTTGACCACCTACATACCAAAGACGAGCGCCTT11.9607843137254901No Hit
AGGCCCCTTACTTGAGGATAAATTATGTCTAATATTCAAACTGGCGCCGA11.9607843137254901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCTA50.0145.01
TATGTTG50.0145.0145
TATTGAG50.0145.06
TTGAGGC50.0145.08
GCTATTG50.0145.04
TGCTATT50.0145.03
ATTGAGG50.0145.07
TGAGGCT50.0145.09
CTGCTAT50.0145.02
CTATTGA50.0145.05