FastQCFastQC Report
Mon 16 Jul 2018
000000000-BYNJT_l01n03.333000000ee151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BYNJT_l01n03.333000000ee151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9364317
Sequences flagged as poor quality0
Sequence length251
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG94922610.136628223927064No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA5544525.920901652517744No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG5238885.594513726948799No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG5040955.383147537615397No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG3264773.4863941491942234No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG2208262.358164508954577No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG2125022.2692738829751278No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAA2106962.2499879062188946No Hit
CCTGTTTGATCCCCACGCTTTCGCACCTCAGCGTCAGTTACAGACCAGAG1300701.3889961221944964No Hit
CCTGTTTGCTCCCCATGCTTTCGCGCCTCAGCGTCAGTTATAGACCAGAA1161211.2400370470158155No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG1133531.210478030592087No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAG1065531.1378619497823494No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAATAATTTGTTAGTA959391.0245167907066794No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA902310.9635619981681526No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTATGGTCCAGAA855380.9134462235740205No Hit
CCTGTTTGATACCCACACTTTCGAGCATCAGTGTCAGTTGCAGTCCAGTG832610.8891305153381716No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCATCAGCGTCAGTTGTAACTTGGTA758950.8104702136845644No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG755210.8064763292400289No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTGCAGACCAGAA642810.6864462191956979No Hit
CCCATTCGCTCCCCTAGCTTTCGTCTCTCAGTGTCAGTGTCGGCCCAGCA626060.6685591698785934No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGAA592580.6328064289152108No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATCAGTCCAGGT540750.5774580249686122No Hit
CCTGTTTGATACCCACGCTTTCGTGCTTCAGTGTCAGTTGCGGTTTGGTA522510.5579798291749414No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTTAGCGTCAGTATCTGTCCAGTA510190.5448235039458831No Hit
CCTGTTTGCTCCCCACGCTTTCGTTCCTCAGTGTCAATATACAACCAGTT508840.5433818611651016No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAACGTCAGTCATCGTCCAGCA428840.4579511778595278No Hit
CCTATTTGCTCCCCACACTTTCGAGCCTAAGCGTCAGTGATAGTCCAAGT361300.38582632347879725No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAA339110.36212998769691374No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTAATGTCCAGCA336410.3592467021353506No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAG335520.3582962857835761No Hit
CCTGTTCGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTACAGTCCAGAA324620.34665635518319166No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAATGTTGGCCCAGGT324300.3463146324499694No Hit
CCTGTTCGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG308660.3296129338637297No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGTGTCAGTTACAGTCCAGAA258770.27633622398729135No Hit
CCTGTTTGCTACCCACGCTTTCGGGCATGAACGTCAGTATTATCCCAGGG250710.26772908264425477No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCCTCAGCGTCAGTTTCAGTCCAGAA248000.2648351182472785No Hit
CCTGTTCGCTACCCATGCTTTCGAGTCTCAGCGTCAGTTGCAGACCAGGT243620.26015778833629827No Hit
CCTGTTCGCTCCCCACGCTTTCGCTCCTCAGCGTCAGTTACAGACCAGAG232330.2481013831547992No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAG229580.2451647034161701No Hit
CCTGTTTGCTACCCACGCTTTCGCGCTTCAGCGTCAGTATCTGTCCAGTA221380.2364080583773488No Hit
CCTGTTTGCTACCCACGCTTTCGTGCCTCAGTGTCAGTTGCAGTCCAGAA174680.1865378969977202No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTGCAGGCCCAGGG172320.18401769184020575No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTACAGGCCCAGGG172010.18368664794239667No Hit
CCTGTTTGCTCCCCATGCTTTCGTACCTCAGCGTCAGTATTAGGCCAGAT170460.18203142845335119No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGTGTCAGTATGATGCCAGGA160520.17141666605263364No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGTCCAGTG159620.17045557086544594No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACACTCCCAAGG146990.15696820173857848No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAATTACAGACCAGAG145480.15535569759118578No Hit
CCTGTTCGCTCCCCACGCTTTCGTGACTCAGCGTCAGTTTCAGTCCAGTA137630.14697281179182634No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTTACTGCCCAGAG137180.1464922641982325No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATATCTTGGTA129010.1377676556656508No Hit
CCTGTTTGATACCCGCACTTTCGAGCATCAGTGTCAGTGCCGGCCCGGTG125660.1341902458022299No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAG120380.12855182070406204No Hit
CCCGTTCGCTCCCCTGGCTTTCGCGCCTCAGCGTCAGTTGTCGTCCAGAA112230.1198485698423067No Hit
CCTGTTCGCTCCCCATGCTTTCGCTCCTCAGCGTCAGTAACAGCCCAGAG106620.11385774317550335No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTCACAGTCCAGAG106220.11343058975897549No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGTCCAGAG106170.11337719558190949No Hit
CCTGTTCGCTCCCCACGCTTTCGTCCCTCAGCGTCAGTTATAACTTGGTA102880.10986385873096778No Hit
CCTGTTTGCTACCCACGCTTTCGCACATGAGCGTCAGTATTGCCCCAGAA102680.10965028202270385No Hit
CCTGTTTGCTACCCACACTTTCGAGCCTCAACGTCAGTTACAGTCCAGAA101000.1078562376732868No Hit
CCTGTTTGCTACCCACGCTTTCGAACCTCAGCGTCAGTTACAGACCAGAG99390.10613694517176214No Hit
CCTGTTTGATACCCACACTTTCGAGCCTCAGCGTCAGTTGTGCTCCCGGC94340.1007441332880978No Hit
CCTGTTCGCTACCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG94190.10058395075689984No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGC5340800.0244.763753
TTTGCTC5158400.0244.612925
CTGTTCG616900.0244.324862
CTCCCCA5698350.0243.553229
CCTGTTC616850.0243.530441
TGTTTGA590700.0243.423923
TTTGATC361700.0243.069535
GTTTGCT5410400.0241.803014
GCTCCCC5850200.0241.771158
CTGTTTG6003950.0241.3052
TGATCCC363550.0241.158727
TTGATAC233050.0240.637226
CCCTCCG1619900.0240.19803245
TTGATCC366550.0239.819966
TTGCTCC5270800.0239.656856
ATTCGCT76100.0237.273354
TGCTCCC5330800.0236.699757
CCATTCG75100.0236.354862
CTATTTG64650.0236.283842
CCTGTTT6129750.0235.785141
Produced by FastQC (version 0.11.2)