FastQCFastQC Report
Mon 16 Jul 2018
000000000-BYNJT_l01n02.332000000ee154.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BYNJT_l01n02.332000000ee154.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9364317
Sequences flagged as poor quality0
Sequence length12
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAA964521.0299950332736494No Hit
TAGAACTCACCT722550.7715992527805284No Hit
TACCCAGAGATC679710.7258511218703937No Hit
TATGCACCAGTG665970.7111784020126615No Hit
AAGAGATGTCGA664270.709362999992418No Hit
ATCCCGAATTTG663750.7088077005509318No Hit
ACCCGTATGATG663520.7085620873364282No Hit
CGGAGCTATGGT662100.7070456927077543No Hit
CATCCAAATGCG637350.6806155750600925No Hit
TTATCACGTGCA596660.637163393763795No Hit
TAACGCTTGGGT595250.6356576779705343No Hit
ACACGTAAGCCT558570.5964877096749288No Hit
ATCTCTGGCATA553800.591393905182834No Hit
AAGTGGACTCTC550470.5878378529902395No Hit
ATTATACCTCGG544900.5818897416650889No Hit
CTGGCCTAGGAA544220.5811635808569915No Hit
TACCTCTCAGAA533730.5699614825085482No Hit
CCTAATGGAACC533250.5694488984087147No Hit
ACCACATACATC527210.5629988818191439No Hit
GCGACTTGTGTA524910.5605427496741087No Hit
TGAGTCACTGGT523700.5592506105891119No Hit
GCACTACCGAAT522210.5576594641125455No Hit
CCTCTACCTACG513020.5478456143678178No Hit
GACACATTTCTG511080.5457739202976576No Hit
GTACCTAATTGC508750.5432857516463827No Hit
TGGAGCACGTTG502870.5370065964234231No Hit
CCTCGTTCGACT502500.5366114795131348No Hit
GATAGAAAGCCA501160.5351805155677665No Hit
TGTTTGAGCTGT499270.5331622156746723No Hit
CAAGCATGCCTA499150.5330340696497139No Hit
ACCGGCTAGAGT493090.5265626953893168No Hit
GACGGAACCCAT491690.5250676584314692No Hit
CTTCGGCAGAAT491240.5245871108378753No Hit
CAGCATGTGTTG484370.5172507509090092No Hit
CACGAGGTCATT482730.5154994219012449No Hit
AGCCGGCACATA471000.5029731479615652No Hit
GACTGATCATCT468800.5006238041706619No Hit
ACTTATGCGGTT468450.5002500449312No Hit
TGATTTGGACCT468000.49976949733760617No Hit
TACGTCCCGTTC466480.4981463143548003No Hit
CCTGAACTAGTT456700.4877024133206938No Hit
ATTCATGGACGC454950.48583361712338446No Hit
ACGGGACATGCT453930.4847443759112384No Hit
CATTTGGACGAC433950.46340806275567137No Hit
TTATGCAGTCGT433180.46258579242885517No Hit
AGAGCCTACGTT430880.46012966028381996No Hit
TTCCGTAGGGAT424400.4532097749360685No Hit
TCGCATGAAGTC421210.44980322643925874No Hit
GAGGCTCATCAT419370.4478383207232305No Hit
GGCCACGTAGTA418430.4468345101943901No Hit
GTTGGTCAATCT415980.44421819551815683No Hit
CACGGTTGTGAG412870.4408970777046527No Hit
GTCAATTGACCG412520.4405233184651908No Hit
AATGTCCGTGAC410160.43800311330767633No Hit
TAGGATTGCTCG408070.43577123670631823No Hit
CTGGAAATCTGC407070.43470335316499853No Hit
TCGTCGATAATC406490.43408398071103316No Hit
TATCAGGTGTGC405530.4330588125113663No Hit
CCTTGGCTATCC403940.431360877680668No Hit
ATCGGCGTTACA403760.4311686586432305No Hit
GCTCAGTGCAGA401750.42902221272517793No Hit
GAGGCCATCAGT399570.42669422660510103No Hit
TCCAGGCTTAAT394420.4211946263673047No Hit
ATTCTGTGAGCG388410.4147766462839735No Hit
CTCCTGAAAGTT385860.4120535432536083No Hit
GACTCGAATCGT384120.4101954258917121No Hit
AGCTGGAAGTCC383330.40935179789406956No Hit
TACGATGACCAC383170.4091809365274584No Hit
GTCATATCGTAC382370.4083266296944027No Hit
CGAGATACCCAG378890.40461039497061024No Hit
CGGGTTTGACGA376460.40201543796520345No Hit
CCATACATAGCT374680.40011460526165443No Hit
TAGGAACTGGCC373150.3984807434434353No Hit
AATCAGTCTCGT372430.3977118672936852No Hit
CCATAGGGTTCA369650.3947431510488165No Hit
TTCTGGGAACAC362260.3868514916784641No Hit
TAACACCACATC360690.3851749145185922No Hit
TGCGGCATCGAA360680.38516423568317903No Hit
GTCCGAAACACT359490.3838934542690086No Hit
CAAGTTTACGGC358860.38322068763797723No Hit
TAATCCACAGCG356570.3807752343283552No Hit
ATTAGTTCGCGT352930.3768881382379516No Hit
CACGCCATAATG352840.37679202871923284No Hit
AACACAAGGAGT351930.3758202546966319No Hit
GACTTGGTATTC348570.37223216599779785No Hit
TCAACAGCATCG347840.37145261101263444No Hit
TCCCAGAACAAC345180.36861204079272414No Hit
GAAGTTGGAAGT344620.3680140260095851No Hit
GCACACACGTTA344000.3673519382139669No Hit
CCATGCGATAAC340240.36333669609860497No Hit
TTACTGTGCGAT339390.36242899508848325No Hit
CTAACCTCCGCT335930.3587341180355172No Hit
TAAAGTCACCCT334860.35759148264630514No Hit
GGTAGGAACAAT334400.3571002562172981No Hit
TCTTCCGCTACT332150.3546975182493288No Hit
ATTCCTGTGAGT331770.35429172250362734No Hit
TACAGATGGCTC330680.3531277294435889No Hit
TGCAATGTTGCT329440.3518035538523525No Hit
GACTTTCCCTCG326150.3482902170014108No Hit
ACGTGTACCCAA323610.3455777928064588No Hit
GAACTAGTCACC320500.34225667499295465No Hit
CTAGATTTGCCA319740.3414450835015517No Hit
TCGAGGACTGCA316900.3384122942442038No Hit
TCTGTTGCTCTC315890.33733373186747095No Hit
TAGCTCGTAACT307690.32857708682864967No Hit
AGCTTGACAGCT306470.32727426890823963No Hit
ATGGATACGCTC303930.3245618447132877No Hit
GATTGGTTGCAC303600.32420944314465217No Hit
AAGGAGCGCCTT302170.3226823696805651No Hit
TAGCGGATCACG301050.321486340114287No Hit
TGTTATCGCACA300890.3213154787476759No Hit
AATCTTGCTGCA298950.31924378467751574No Hit
CCTCTCGTGATC298280.31852830270483157No Hit
CTAGCGAACATC295640.3157090901557476No Hit
GTGCCGGTGATA291020.3107754681948507No Hit
CGCAAATTCGAC288370.3079455768103536No Hit
AATCCGTACAGC285480.30485939337593976No Hit
TAAGGCCTATCG283400.30263819560999483No Hit
GTAAGCACCTAC278590.2975016757762472No Hit
ATGAGACTCCAC278080.2969570551701742No Hit
GAGTGGTAGAGA276930.29572898909765655No Hit
GGAGACAAGGGA276740.29552609122480583No Hit
TCCAAAGTGTTC272960.29148949143861747No Hit
CAACACGCACGA272220.2906992576180409No Hit
CAACGGGTAGTC269100.2873674609691236No Hit
TCGGAGTGTTGT265880.2839288759660742No Hit
AGTTTGCAACGC260070.2777244725910069No Hit
GAATCTTCGAGC258900.2764750488476629No Hit
TAAACCGCGTGT255870.2732393617174643No Hit
TGAGGATGATAG255610.27296171199672115No Hit
GACAGGAGATAG251430.26849795879400495No Hit
GCCTGAATTTAC251060.26810284188371664No Hit
ATGTGCACGACT243320.2598374232739024No Hit
TTCGCCCTTCAG242890.2593782333511349No Hit
CCAGTGTATGCA239790.25606779437304394No Hit
AGGCATCTTACG239650.2559182906772592No Hit
GTCTACACACAT239470.2557260716398217No Hit
TACACGATCTAC239180.255416385412839No Hit
TCAAGCTCAAGC236110.25213798294098755No Hit
ATGTCGAGAGAA233640.249500310593928No Hit
ACGCGCAGATAC227150.24256974641076332No Hit
TTCCTAGGTGAG226880.242281417854607No Hit
CGTAACCAACCA226410.2417795125901868No Hit
AAGGTGTAAGGT225810.24113878246539497No Hit
TAACTCTGATGC225530.24083977507382545No Hit
GCACGACAACAC224840.24010293543031488No Hit
ATACTTCGCAGG223620.23880011750990487No Hit
TCCTCTGTCGAC223400.23856518313081454No Hit
CTATTTGCGACA222360.23745458424784208No Hit
TGGCAAGACTCT216520.23121814436653523No Hit
GAGGAATAGCAG210290.22456522990411368No Hit
AATGGAGCATGA210120.22438368970208933No Hit
GGTCAGCTTAAC209300.2235080251982072No Hit
TATCGTTGACCA207580.22167126550713734No Hit
TGTAATTGTCGC207180.22124411209060948No Hit
GTCGTCCTAAAT206520.2205393089533385No Hit
GCATGGCTCTAA205420.2193646370578869No Hit
ACTCACGGTATG203530.21734633716479268No Hit
ACACGAGCCACA200670.21429219023661844No Hit
TCTACGGAGAGC198760.21225253267269784No Hit
ATACCTTCGGTA193220.2063364578537869No Hit
GGAAACCACCAC192300.2053540049957728No Hit
ATGTGGGACCCA191240.20422204844197392No Hit
AGAACACGTCTC179720.1919200300459713No Hit
TTGCAGACAGGC179260.19142880361696427No Hit
ACACCTGGTGAT168250.1796714058270347No Hit
CTCTACCTCTAC167970.17937239843546518No Hit
CCATTCGCCCAT167610.17898796036059011No Hit
ATCGCTCGAGGA167110.17845401858993026No Hit
ATTGGGCTAGGC164580.1757522732303915No Hit
AGCCTAAGCACG164020.1751542584472525No Hit
CAGGCGTATTGG160090.17095747612986617No Hit
AGTTCCCGAGTA159430.1702526729925952No Hit
TACTTCGCTCGC159210.17001773861350486No Hit
GGTAACAGCTCG157940.16866152651602886No Hit
TCTAGCGTAGTG151950.16226490410352404No Hit
CAGCGGTGACAT147810.1578438662424606No Hit
CACATGCCTAAG146680.15663715784076937No Hit
ATGGACCGAACC143350.15308110564817487No Hit
GCGATATATCGC138320.14770965143533693No Hit
AGGCTACACGAC131000.13989274391287693No Hit
TGCTATATCTGG130980.13987138624205053No Hit
TTACGAGACGGC127260.13589885946834135No Hit
CAGCTAGAACGC118770.12683252820253735No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGGTG15500.06.00000052
TCGCAGG22300.06.06
CAACGGG26550.06.01
ACACCTG18550.06.01
GGCGTGT600.00339616026.03
AGGCGTA15750.06.02
CTTGCTG30600.06.04
GCAGGAA550.0091597836.02
GCTGTCG806.5380285E-56.05
TATCGCC704.6966344E-46.04
GCTCAGT41250.06.01
GTGCCGG30200.06.01
GAGTGGT27650.05.99999951
GTCCGTG41500.05.99999954
ATCGGCG42400.05.99999951
GCCGGCA50150.05.9940182
AGCCGGC50000.05.9941
TACGTCC49100.05.993891
ACGAGGT47850.05.99373052
CGAGGTC47700.05.99371053
Produced by FastQC (version 0.11.2)