FastQCFastQC Report
Thu 27 Sep 2018
000000000-BY879_l01.3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY879_l01.3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12911481
Sequences flagged as poor quality0
Sequence length251
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTTTGCTCCCCACGCTNTCGTGCATGAGCGNCAGTATTGGCCCAGGG135219410.472803236127598No Hit
CCTGTTTGCTCCCCACGCTTTCGTGCATGAGCGTCAGTATTGGCCCAGGG3739132.8959729716521285No Hit
CCTGTTTGCTCCCCACGCTNTCGCACCTGAGCGNCAGTCTTTGTCCAGGG1562011.20978375757204No Hit
CCTGTTTGCTCCCCACGCTNTCGCGCCTCAGCGNCAGTTACAGACCAAAA909170.7041562466768916No Hit
CCTGTTTGCTCCCCACGCTNTCGCACCTCAGTGNCAGTATTAGCCCAGGT868250.672463522968434No Hit
CCTGTTTGCTCCCCACGCTNTCGAGCCTCAGCGNCAGTTACAGTCCAGAG828180.6414291280760124No Hit
CCTGTTTGCTCCCCACGCTNTCGCACATGAGCGNCAGTACATTCCCAAGG792740.6139806889697627No Hit
CCTGTTTGCTCCCCACGCTNTCGAGCCTCAGCGNCAGTTACAGACCAGAG728920.5645518124528084No Hit
CCTGTTTGCTCCCCACGCTNTCGCACATGAGCGNCAGTATATTCCCAAGG710970.5506494568671092No Hit
CCTGTTTGATCCCCACGCTNTCGCACATCAGCGNCAGTTACAGACCAGAA593800.4599007658377842No Hit
CCTGTTTGCTCCCCACGCTNTCGCACCTGAGCGNCAGTCTTCGTCCAGGG569580.4411422671032084No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTTGTCCAGGG438870.3399067852866762No Hit
CCTGTTTGCTCCCCACGCTNTCGCGCCTCAGCGNCAGTTACAGACCAGAA422550.327266872018787No Hit
CCTGTTTGCTCCCCATGCTNTCGTACCTCAGCGNCAGTATTAGGCCAGAT407840.31587391097891865No Hit
CCTGTTTGCTCCCCACGCTNTCGCGCATGAGCGNCAGTATTGGCCCAGGG301990.23389261077021295No Hit
CCTGTTTGCTCCCCACGCTNTCGCACCTCAGTGNCAGTATCAGTCCAGGT300130.2324520324198285No Hit
CCTGTTTGATACCCACACTNTCGAGCATCAGTGNCAGTTGCAGTCCAGTG298770.23139870631417112No Hit
CCTGTTTGCTCCCCACGCTNTCGTGCATGAGCGNCAGTACAGGTCCAGGG294430.22803735682994072No Hit
ACTGTTTGCTCCCCACGCTNTCGTGCATGAGCGNCAGTATTGGCCCAGGG258180.1999615690872333No Hit
CCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAAAA258030.19984539341381521No Hit
CCTGTTTGCTCCCCACGCTNTCGTGCCTCAGTGNCAGTGTTGGTCCAGGT255970.19824991416553997No Hit
CCTGTTTGATACCCACGCTNTCGTGCTTCAGTGNCAGTTGCGGTTTGGTA255430.19783168174123478No Hit
CCTGTTTGCTACCCACGCTNTCGCGCTTTAGCGNCAGTATCTGTCCAGTA246820.19116319808703586No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTCAGTGTCAGTATTAGCCCAGGT242990.18819684589242705No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGTCCAGAG233640.18095522891603216No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTACATTCCCAAGG220860.17105706154081007No Hit
CCTGTTTGCTCCCCACGCTTTCGAGCCTCAGCGTCAGTTACAGACCAGAG209900.16256849233639425No Hit
CCTGTTTGATACCCGCACTNTCGAGCATCAGTGNCAGTGCCGGTCCAGTG206320.15979576626414893No Hit
CCTGGTTGCTCCCCACGCTNTCGTGCATGAGCGNCAGTATTGGCCCAGGG202810.1570772555061654No Hit
CCTGTTTGCTCCCCACGCTTTCGCACATGAGCGTCAGTATATTCCCAAGG201300.15590775372708987No Hit
CCTGTTTGCTCCCCACGCTNTCGCGCCTCAGCGNCAGTTACAGACCAGAG197390.15287944117332472No Hit
CCCATTTGCTCCCCTAGCTNTCGTCTCTCAGTGNCAGTGTCGGCCCAGCA196280.15201974119003078No Hit
CCTGTTTGCTACCCACGCTNTCGAACCTCAGCGNCAGTTACAGACCAGAG174980.13552279556466063No Hit
CCTGTTTGATCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCAGAA174060.13481025143436295No Hit
CCTGTTTGCTCCCCACGCTNTCGTGCCTGAGCGNCAGTATTGGCCCAGGG168360.13039557584447514No Hit
CCTGTTTGATACCCGCACTNTCGAGCATCAGTGNCAGTGCCGGCCCGGTG164110.12710393176429566No Hit
CCTGTTCGCTCCCCACGCTNTCGAGCCTCAGCGNCAGTTACAGACCAGAG162490.1258492344913802No Hit
CCTGTTTGCTCCCCACGCTNTCGTGCCTCAGTGNCAGTTACAGTCCAGAG159300.12337856517002195No Hit
CCTGTTCGCTCCCCACGCTNTCGCTCCTCAGCGNCAGTTACAGACCAGAG156880.12150426430554326No Hit
CCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCAGTCTTCGTCCAGGG156560.12125642286891797No Hit
CCTGTTTGATCCCCACGCTNTCGCACATCAGCGNCAGTTGCAGACCAGAA153450.11884771390671604No Hit
CCTGTTTGCTCCCCACGCTNTCGTGCCTCAGCGNCAGTTTCAGTCCAGAA152450.11807320941726206No Hit
CCTGTTTGCTACCCACGCTNTCGCGCTTCAGCGNCAGTATCTGTCCAGTA146500.11346490770501076No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGCTC5355950.0337.509435
TTGCTCC5462000.0336.92996
CTCCCCA5681700.0335.276559
TGCTCCC5518650.0333.569247
TTGATAC242450.0333.22826
GCTCCCC5856800.0331.80228
TTTGATC203250.0330.37385
TGATCCC212100.0323.443367
TGTTTGC5893650.0318.770783
GTTTGCT5936450.0316.502174
TGTTTGA465350.0316.45543
TTGATCC217100.0315.604836
GTTGCTC107550.0309.658265
ATACCCG90650.0305.28669
ATACCCA176500.0300.27549
TTCGCTC356350.0296.408845
CTACCCA376600.0295.55379
ATTTGCT59200.0295.223664
TGTTCGA20750.0294.87383
GATACCC275350.0294.14948
Produced by FastQC (version 0.11.5)