FastQCFastQC Report
Thu 27 Sep 2018
000000000-BY879_l01.2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY879_l01.2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12911481
Sequences flagged as poor quality0
Sequence length12
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCGTTCGACT4180363.237707587533917No Hit
ATTCCTGTGAGT3576732.7701934425647994No Hit
GCACACACGTTA3379842.6177012536362017No Hit
CACGCCATAATG3350372.5948766063319924No Hit
GACTTTCCCTCG3289672.5478641838221345No Hit
GAGGAATAGCAG3155762.44415028763935No Hit
TCCAAAGTGTTC2979532.3076593614628713No Hit
CACGGTTGTGAG2967712.2985047183975253No Hit
GAGTGGTAGAGA2750512.130282343288117No Hit
ACGGGACATGCT2665332.0643100508764256No Hit
CAGCGGTGACAT2664672.063798877913386No Hit
AGGCTACACGAC2611292.0224558282663314No Hit
TACAGATGGCTC2508391.9427593163015149No Hit
GGCCACGTAGTA2485521.925046398627702No Hit
CTTCGGCAGAAT2431161.8829443345809826No Hit
TTCCTAGGTGAG2389681.8508178883584308No Hit
TCCTCTGTCGAC2367941.8339801607577009No Hit
TACACGATCTAC2266431.7553602100332255No Hit
GCGATATATCGC2187871.6945151373417195No Hit
CAAGCATGCCTA1951451.511406785945005No Hit
ACACCTGGTGAT1864301.443908719689089No Hit
ACGTGTACCCAA1840971.4258395299501272No Hit
GAGGCTCATCAT1826431.4145782346734663No Hit
CGGAGCTATGGT1705421.3208554464046378No Hit
CCTGAACTAGTT1601341.2402450191422658No Hit
CAGCTAGAACGC1599081.2384946389960996No Hit
CTAGCGAACATC1596771.2367055336254609No Hit
GTTGGTCAATCT1558531.20708848194874No Hit
ACACGTAAGCCT1534071.1881441021366952No Hit
GAAGTTGGAAGT1463911.1338048671566026No Hit
GTCATATCGTAC1412891.0942896481046598No Hit
TGAGTCACTGGT1319771.0221677900467034No Hit
GACAGGAGATAG1317511.0204174099005374No Hit
TCCCAGAACAAC1310981.0153598955844028No Hit
ATCCCGAATTTG1278490.9901962447220424No Hit
CCAGTGTATGCA1205470.9336419269021113No Hit
TTACTGTGCGAT1194480.9251301225630119No Hit
TATCGTTGACCA1051000.8140042184161523No Hit
GACGGAACCCAT1017840.7883216495458577No Hit
GAACTAGTCACC978650.7579688186041554No Hit
ATGTGCACGACT960920.744236854006136No Hit
TAGGAACTGGCC890630.6897969334424146No Hit
AATCAGTCTCGT877900.6799374912916651No Hit
TCGAGGACTGCA861540.6672665978441977No Hit
GAATCTTCGAGC741970.5746590960401832No Hit
ATGAGACTCCAC738360.5718631348332542No Hit
TAGGATTGCTCG711130.5507733775854219No Hit
AGCTGGAAGTCC575640.4458357643092996No Hit
GACTTGGTATTC562710.43582142126065937No Hit
GGAGACAAGGGA531310.41150198029180385No Hit
AATCCGTACAGC498110.38578843124193113No Hit
ACGCGCAGATAC457670.3544674696884114No Hit
AGGCATCTTACG451790.34991338329042193No Hit
AAGAGATGTCGA417820.32360346578366955No Hit
GTCAATTGACCG264730.20503457349315699No Hit
TCTAGCGTAGTG213760.16555807966568667No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTTA2050.06.00000053
GGCACCC600.00339643686.00000054
CGCGCCG600.00339643686.00000052
GCGTTGA2050.06.00000053
CGGGGCT600.00339643686.00000051
GGCCAAG600.00339643686.00000051
GCACAGC600.00339643686.00000056
ATCCCGG650.00126191466.00000051
GTCCGTC650.00126191466.00000055
CGGGTCA909.15167E-66.02
CAGCGGG1001.2864839E-66.01
CGGCGCT3850.06.01
GGCAGAC2000.06.05
GCCCACG852.444796E-56.01
CGAACAG550.0091604166.05
GGTCGAG2100.06.05
GCAACTG1054.83029E-76.03
CTCCCGA1001.2864839E-66.01
ACCTGGG1101.8151331E-76.03
AAGCGGT751.7512625E-46.01
Produced by FastQC (version 0.11.5)