FastQCFastQC Report
Mon 22 Apr 2019
000000000-BY6FC_l01_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename000000000-BY6FC_l01_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1800259
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATAGCT25950.0138.004032
ATAGCTC26200.0136.967683
GCATAGC27550.0130.018161
TAGCTCT27900.0128.88544
CTTAAAC62600.057.9099
TCTTAAA66550.054.6897668
GCTCTTA68150.053.0836756
CTCTTAA70500.051.931227
AGCTCTT90300.040.142885
CCGATCT951.0297193E-438.1547472
CTTAGGC900.003628983632.222244145
TCCGATC1255.1512447E-429.0040511
CCCCATA25550.027.07032235-39
TAAACCC9400.026.99515210-14
CCCATAT26600.026.8194235-39
AACCCGT3550.026.54975910-14
GATATCC3500.025.68573620-24
GCTTAGC2400.025.37502120-24
AAACCCG3850.025.23420710-14
CCGGATC351.2568111E-424.857162120-124
Produced by FastQC (version 0.11.5)